biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 53:779-783, 2009 | DOI: 10.1007/s10535-009-0143-7

The effect of phenyl acetic acid on shoot bud induction, elongation and rooting of chickpea

S. Kiran Ghanti1,2,*, K. G. Sujata2, M. Srinath Rao1
1 Plant Tissue Culture Laboratory, Department of Botany, Gulbarga University, Gulbarga, India
2 Department of Crop physiology, University of Agricultural Science, GKVK, Bangalore, India

A highly efficient protocol for plant regeneration from cotyledonary node of two chickpea (Cicer arietinum L.) cultivars ICCV-10 and Annigeri used phenylacetic acid (PAA). The Murashige and Skoog (MS) medium supplemented with 2.0 mg dm-3 6-benzylaminopurine (BAP) and 1.0 mg dm-3 PAA was used for induction of bud formation. Buds were elongated on MS medium supplemented either with only 0.75 mg dm-3 gibberellic acid (GA3) or 0.2 mg dm-3 GA3 + 0.6 mg dm-3 PAA. The elongated shoots were then transferred onto rooting medium containing 1 mg dm-3 PAA. The frequency of multiple shoot induction and rooting was higher in Annigeri as compared to ICCV-10. The complete plantlets with well-developed roots were transferred to pots containing sterilized soil and sand in the ratio 3:1 where they survived (74 %) and set normal seeds.

Keywords: auxins; cytokinins; elongation; gibberellic acid; multiple shoots
Subjects: auxins; chickpea; Cicer arietinum; cytokinins; ex vitro transfer; gibberellic acid, gibberellin; in vitro culture, direct regeneration; in vitro culture, micropropagation; in vitro culture, rooting

Received: January 19, 2009; Accepted: August 15, 2009; Published: December 1, 2009  Show citation

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Kiran Ghanti, S., Sujata, K.G., & Srinath Rao, M. (2009). The effect of phenyl acetic acid on shoot bud induction, elongation and rooting of chickpea. Biologia plantarum, 53(4), 779-783. doi: 10.1007/s10535-009-0143-7
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    References

    1. Abe, U., Uchiyama, M., Sato, R.: Isolation of phenylacetic acid and its p-hydroxy derivative as auxin like substances from Undaria pinnatifida. - Agr. biol. Chem. 38: 897-898, 1974. Go to original source...
    2. Barna, K.S., Wakhulu, A.K.: Somatic embryogenesis and plant let regeneration from callus culture of chickpea (Cicer arietinum L). - Plant Cell Rep. 12: 521-524, 1993. Go to original source...
    3. Batra, P., Yadav, N.R., Sindhu, A., Yadav, R.C., Chowdhury, V.K., Chowdhury, J.B.: Efficient protocol for in vitro direct plant regeneration in chickpea (Cicer arietinum L.). - Indian J. exp Biol. 40: 600-602, 2002.
    4. Chakrabarty, R., Singh, D.T., Garg, G.K.: Transformation studies on chickpea embryo axis. - J. Plant Biochem. Biotechnol. 9: 107-110, 2000. Go to original source...
    5. Chakraborti, D., Sarkar, A., Das, S.: Efficient and rapid in vitro plant regeneration system for Indian cultivars of chickpea (Cicer arietinum L.). - Plant Cell Tissue Organ Cult. 86: 117-123, 2006. Go to original source...
    6. Dhaka, N., Kothari, S.L.: Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Helianthus annuus L. - Plant Cell Rep. 21: 29-34, 2002. Go to original source...
    7. Fiore, M.C., Trabac, T., Sunseri, F.: High frequency of plant regeneration in sunflower from cotyledons via somatic embryogenesis. - Plant Cell Rep. 16: 295-298, 1997. Go to original source...
    8. Gang, W., Wei, Z.M.: High frequency plant regeneration from the cotyledonary node of common bean. - Biol. Plant. 53: 312-316, 2009. Go to original source...
    9. Husain, S., Jain, A., Kothari, S.L.: Phenylacetic acid improves bud elongation and in vitro plant regeneration efficiency in Capsicum annuum L. - Plant Cell Rep. 19: 64-68, 1999. Go to original source...
    10. Jayanand, B., Sudarsanam, G., Sharma, K.K.: An efficient protocol for the regeneration of whole plants of chickpea (Cicer arietinum L.) by using axillary meristem explants derived from In vitro - germinated seedlings. - In Vitro cell. dev. Biol. Plant 39: 171-179, 2003. Go to original source...
    11. Kar, S., Johnson, T.M., Nayak, P., Sen, S.K.: Efficient transgenic plant regeneration through Agrobacteriummediated transformation of chickpea (Cicer arietinum L.). - Plant Cell Rep. 16: 32-37, 1996. Go to original source...
    12. Kiran, G., Kaviraj, C.P., Jogeswar, G., Kavi Kishor, P.B., Srinath Rao, M.: Direct and high frequency somatic embryogenesis and plant regeneration from hypocotyls of chickpea (Cicer arietinum L.), a grain legume. - Curr. Sci. 89: 1012-1018, 2005.
    13. Kumar, V.D., Kirti, P.B., Sachan, J.K.S., Chopra, V.L.: Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.). - Plant Cell Rep. 13: 468-472, 1994. Go to original source...
    14. Kumar, V.D., Kirti, P.B., Sachan, J.K.S., Chopra, V.L.: Picloram induced somatic embryogenesis in chickpea (Cicer arietinum L.). - Plant Sci. 109: 207-213, 1995. Go to original source...
    15. Ma, X.-H., Wu, T.-L.: Rapid and efficient regeneration in soybean [Glycine max (L.) Merrill] from whole cotyledonary node explants. - Acta Physiol Plant. 30: 209-216, 2008. Go to original source...
    16. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962. Go to original source...
    17. Murthy, B.N.S., Jerrin, V., Rana, P.S., Filetcher, R.A., Praveen, K.S.: In vitro regeneration of chickpea (Cicer arietinum L.): stimulation of direct organogenesis and somatic embryogenesis, by thidiazuron. - Plant Growth Regul. 19: 233-240, 1996. Go to original source...
    18. Polisetty, R., Patil, P., Deveshwar, J.J., Khetarpal, S., Chandra, R.: Rooting and establishment of in vitro grown shoot tip explants of chickpea (Cicer arietinum L.). - Indian J. exp. Biol. 34: 806-809, 1996.
    19. Polisetty, R, Paul, V., Deveshwar, J.J., Khetarpal, S., Suresh, K., Chandra, R.: Multiple shoot induction by benzyladenine and complete plant regeneration from seed explants of chickpea (Cicer arietinum L.). - Plant Cell Rep. 16: 565-571, 1997. Go to original source...
    20. Rao, B.G., Chopra, V.L.: Regeneration in chickpea (Cicer arietinum L.) through somatic embryogenesis. - J. Plant Physiol. 134: 637-638, 1991. Go to original source...
    21. Sagare, A.P.; Suhasini K., Krishnamurthy, K.V.: Plant regeneration via somatic embryogenesis in chickpea (Cicer arietinum L.). - Plant Cell Rep. 12: 652-655, 1993. Go to original source...
    22. Sanyal, I., Aditya, K.S., Kaushik, M., Amla, V.D.: Agrobacterium-mediated transformation of chickpea (Cicer arietinum L.) with Bacillus thuringiensis cry1Ac gene for resistance against pod borer insect Helicoverpa armigera. - Plant Sci. 168: 1135-1146, 2005. Go to original source...
    23. Schneider E.A.A., Wightman, F.: Auxins on non-flowering plants. I. Occurrence of 3-indoleacetic acid and phenylacetic acid in vegetative and fertile fronds of the ostrich fern (Matteucia struthiopteris). - Physiol Plant. 68: 396-402, 1986. Go to original source...
    24. Sharma, L., Amla, D.V.: Direct shoot regeneration in chickpea (Cicer arietinum L.). - Indian J. exp. Biol. 36: 605-609, 1998.
    25. Shri, P.V, Davis, T.M.: Zeatin-induced shoot regeneration from immature chickpea (Cicer arietinum L.) cotyledons. - Plant Cell Tissue Organ Cult. 28: 45-51, 1992. Go to original source...
    26. Singh, R., Srivastava, K., Jaiswal, H.K., Amla, D.V., Singh, B.D.: High frequency multiple shoot regeneration from decapitated embryo axes of chickpea and establishment of plantlets in the open environment. - Biol. Plant. 45: 503-508, 2002. Go to original source...
    27. Sing, R.P., Sing, B.D.: Recovery of rare interspecific hybrids of gram Cicer arietinum × C. cuneatum L. through tissue culture. - Curr.Sci. 58: 874-876, 1989.
    28. Small, D.K., Morris, D.A.: Promotion of elongation and acid invertase activity in Phaseolus vulgaris L. internode segments by phenylacetic acid. - Plant Growth Regul. 9: 329-340, 1990. Go to original source...
    29. Subhadra, Vashishat, R.K., Chowdhury, J.B., Singh, M., Sareen, P.K.: Multiple shoots from cotyledonary node explants of non-nodulating genotype (ICC435M) of chickpea, Cicer arietinum L. - Indian J. exp. Biol. 36: 1276-1279, 1998.
    30. Surya Prakash, Chowdhary, J.B., Jain, R.K., Chowhary, V.K.: Factors affecting regeneration of chickpea (Cicer arietinum L.). - Indian J. exp. Biol. 30: 1140-1153, 1992.

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