biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:83-88, 2010 | DOI: 10.1007/s10535-010-0012-4

The protection of wheat plasma membrane under cold stress by glycine betaine overproduction

X. -Y. Zhang1, C. Liang1, G. -P. Wang1, Y. Luo1, W. Wang1,*
1 College of Life Science, State Key Laboratory of Crop Science, Shandong Agricultural University, Tai'an, Shandong, P.R. China

We aimed to study the protection of wheat plasma membrane (PM) under cold stress (0-2 °C) by the overaccumulation of glycine betaine (GB). For this, we used wild-type winter wheat (Triticum aestivum L.) cv. Shi 4185 (WT) and 3 transgenic lines (T1, T4, and T6) expressing the BADH gene isolated from Atriplex hortensis L. Under cold stress, the transgenic lines with higher GB content maintained better membrane integrity and higher plasma membrane H+-ATPase activity than WT. In these transgenic lines, ROS production and membrane lipid peroxidation were lower, while antioxidative enzyme activities and compatible solute contents were higher in comparison with WT. This may be attributable to their enhanced cold-stress tolerance mediated by GB overproduction.

Keywords: antioxidative enzymes; BADH gene; compatible solutes; H+-ATPase; lipid peroxidation; ROS production; Triticum aestivum
Subjects: Atriplex hortensis; electrolyte leakage; fatty acids; glycinebetaine; hydrogen peroxide; malondialdehyde; plasma membrane ATPase; proline; reactive oxygen species (ROS); sugars; superoxide; temperature low; Triticum aestivum; wheat

Received: April 5, 2008; Accepted: August 8, 2008; Published: March 1, 2010  Show citation

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Zhang, X.-Y., Liang, C., Wang, G.-P., Luo, Y., & Wang, W. (2010). The protection of wheat plasma membrane under cold stress by glycine betaine overproduction. Biologia plantarum, 54(1), 83-88. doi: 10.1007/s10535-010-0012-4
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