biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 54:126-130, 2010 | DOI: 10.1007/s10535-010-0019-x

In vitro culture of Capparis decidua and assessment of clonal fidelity of the regenerated plants

P. Tyagi1, S. Khanduja1, S.L. Kothari1,*
1 Department of Botany, University of Rajasthan, Jaipur, India

A protocol for in vitro multiplication of Capparis decidua (Forsk.) Edgew. has been developed from cultured leaves procured from multiplying axillary shoots on the cultured nodal explants. The highest efficiency of shoot formation was observed on Murashige and Skoog (MS) medium containing 2 mg dm-3 benzyladenine (BA) and 0.5 mg dm-3 1-naphthaleneacetic acid. The regenerated shoots were transferred to MS medium containing 3 mg dm-3 BA for growth and proliferation. Shoots above 2 cm in length were transferred to MS medium supplemented with 1 mg dm-3 indole-3-butyric acid plus 0.5 mg dm-3 indole-3-acetic acid for root induction. No variation was detected among the micropropagated plants by randomly amplified polymorphic DNA (RAPD) markers.

Keywords: adventitious shoot regeneration; growth regulators; leaf explant; micropropagation; RAPD; root induction
Subjects: auxins; Capparis decidua; cytokinins; genetic stability; in vitro culture, direct organogenesis; random amplified polymorphic DNA (RAPD)

Received: December 16, 2007; Accepted: June 5, 2008; Published: March 1, 2010  Show citation

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Tyagi, P., Khanduja, S., & Kothari, S.L. (2010). In vitro culture of Capparis decidua and assessment of clonal fidelity of the regenerated plants. Biologia plantarum, 54(1), 126-130. doi: 10.1007/s10535-010-0019-x
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