biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:181-184, 2010 | DOI: 10.1007/s10535-010-0031-1

Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis

P. Kumar1, V. K. Singhal1,*, D. Kaur1, S. Kaur1
1 Department of Botany, Punjabi University, Patiala, India

Present cytological investigations from the cold desert regions of Lahaul-Spiti and Kinnaur (India) record the first ever tetraploid (2n=32) chromosome count and cytomixis in Clematis orientalis L. var. acutifolia Hook. f. et Thoms. The phenomenon of cytomixis (9.33-29.80 %) involving chromatin transfer among 2-3 proximate pollen mother cells (PMCs) during male meiosis occurs through narrow and broad cytoplasmic channels from early prophase to tetrad stage. However, frequency of its occurrence during the later meiotic stages is rather low. Chromatin transfer results into the formation of hypo-, hyperploid and enucleated PMCs. Various meiotic abnormalities associated with cytomixis such as chromatin stickiness, pycnotic chromatin, interbivalent connections, out of plate bivalents, late disjunction of bivalents, and laggards and bridges resulted into some pollen sterility (16.33-49.30 %) and heterogeneous pollen grains size.

Keywords: chromatin stickiness; coenocytes; heterogeneous size of pollen grains; laggards and bridges; micronuclei; morphovariants
Subjects: Clematis orientalis; cytomixis; meiotic abnormalities; pollen fertility

Received: December 17, 2008; Accepted: July 14, 2009; Published: March 1, 2010  Show citation

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Kumar, P., Singhal, V.K., Kaur, D., & Kaur, S. (2010). Cytomixis and associated meiotic abnormalities affecting pollen fertility in Clematis orientalis. Biologia plantarum, 54(1), 181-184. doi: 10.1007/s10535-010-0031-1
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