biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:237-244, 2010 | DOI: 10.1007/s10535-010-0042-y

Ex vitro acclimatization of plantain plantlets micropropagated in temporary immersion bioreactor

C. Aragón1,2, L. Carvalho1, J. González2, M. Escalona2, S. Amâncio1,*
1 Departamento de Botânica e Engenharia Biologica, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisboa, Portugal
2 Laboratorio de Células y Cultivo Tejidos, Centro de Bioplantas, Universidad de Ciego de Ávila, Ciego de Ávila, Cuba

Plantain (Musa ABB CEMSA 3/4) plantlets were micropropagated in temporary immersion bioreactors (TIB) or in gelled medium (GM). After ex vitro transfer ROS accumulation was determined by infiltrating leaves with nitroblue tetrazolium (NBT) and 3,3'-diaminobenzidine (DAB). Stomatal cells were more stained with NBT and DAB in GM plants than in TIB plants, but the difference disappeared at the end of acclimatization. At the end of the in vitro phase, GM plantlets showed higher activities of ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR), while activities of catalase (CAT), superoxide dismutase (SOD) and glutathione transferase (GT) were higher in TIB grown plantlets. At the end of acclimatization GT, SOD, CAT and MDHAR stabilized at low values of activity in plantlets derived from both treatments. Concerning the correspondent genes, GM plantlets showed higher expression of all transcripts with the exception of CuZnSOD. The immunobloting of peroxiredoxins (PRXs) showed that chloroplast-located PRXs were expressed at higher levels in TIB plantlets, some showing polymerization. In conclusion, TIB grown plantlets had an improved anti-oxidative response when compared with GM.

Keywords: ascorbate-glutathione cycle; ex vitro growth; oxidative stress; TIB culture
Subjects: ascorbate peroxidase; catalase; chloroplast; cytoplasm; dehydroascorbate reductase; ex vitro transfer; glutathione reductase; mitochondria; monodehydroascorbate reductase; Musa sp.; peroxidase; plantain; polymerase chain reaction (PCR); reactive oxygen species (ROS); stomata number and size; superoxide dismutase (SOD); temporary immersion bioreactor

Received: July 5, 2008; Accepted: March 25, 2009; Published: June 1, 2010  Show citation

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Aragón, C., Carvalho, L., González, J., Escalona, M., & Amâncio, S. (2010). Ex vitro acclimatization of plantain plantlets micropropagated in temporary immersion bioreactor. Biologia plantarum, 54(2), 237-244. doi: 10.1007/s10535-010-0042-y
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