biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:252-258, 2010 | DOI: 10.1007/s10535-010-0044-9

Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization

G. Mirzaghaderi1, G. Karimzadeh1,*, H. S. Hassani2, M. Jalali-Javaran1, A. Baghizadeh3
1 Plant Breeding and Biotechnology Department, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2 Agronomy and Plant Breeding Department, Faculty of Agriculture, University of Shahid Bahonar, Kerman, Iran
3 International Center of High Technology and Environmental Sciences, Mahan, Iran

The new salt tolerant cereal, Tritipyrum (2n=6x=42, AABBEbEb) offers potential to introduce desirable characters for wheat improvements. This study was aimed to generate a segregating population from Iranian local wheat cultivars (2n=6x=42, AABBDD) and Tritipyrum crosses, study of the meiotic behaviour in F2 hybrids and identification of Eb chromosomes in F3 individuals. Results showed meiotic abnormalities in F2 plants and different pairing frequency in the meiosis among F2 plants. Genomic in situ hybridization revealed that total and Eb chromosome number of F3 seeds ranged from 39 to 45 and 0 to 10, respectively. A significant prevalence of hyper-aneuploidy was observed among F3 genotypes. C-banding patterns identified Eb chromosomes in Tritipyrum, indicating that it also can be useful to study wheat-Tritipyrum derivatives.

Keywords: alien chromosomes; meiotic abnormalities; Thinopyrum bessarabicum; Triticum aestivum
Subjects: chromosome characteristics; fluorescence in situ hybridization (FISH); genomic in situ hybridization (GISH); karyogram; meiotic abnormalities; Thinopyrum bessarabicum; Triticum aestivum; Tritipyrum; wheat

Received: July 24, 2008; Accepted: January 15, 2009; Published: June 1, 2010  Show citation

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Mirzaghaderi, G., Karimzadeh, G., Hassani, H.S., Jalali-Javaran, M., & Baghizadeh, A. (2010). Cytogenetic analysis of hybrids derived from wheat and Tritipyrum using conventional staining and genomic in situ hybridization. Biologia plantarum, 54(2), 252-258. doi: 10.1007/s10535-010-0044-9
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