biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:520-524, 2010 | DOI: 10.1007/s10535-010-0091-2

Simple method for isolation of oxygen evolving photosystem 2 core complex free of cytochrome f contamination

R. K. Mishra1,*
1 Department of Biochemistry, University of Lucknow, Lucknow, India

This communication presents a simple method for isolation of oxygen evolving photosystem 2 (PS 2) core complex by solubilisation of PS 2 membranes with the nonionic detergent octyl-β-D-thioglucopyranoside (OTG). This complex was free of cytochrome (Cyt) f contamination and also lacked the 22 and 10 kDa proteins that may not be directly required for primary photochemistry of the PS 2 complex and water oxidation. OTG could also remove the Cyt f contamination from the PS 2-core complex isolated using octyl-β-D-glucopyranoside (OGP). The Cyt f contamination in the PS 2 complexes could potentially interfere with spectrophotometric determinations of redox states of Cyt b559 and its stoichiometry in the PS 2 complex.

Keywords: octyl-β-D-thioglucopyranoside; oxygen evolution; spinach
Subjects: cytochrome; oxygen evolving complex; photosystems (PS 1, PS 2); proteins; spinach; Spinacia oleracea

Received: November 10, 2008; Accepted: June 25, 2009; Published: September 1, 2010  Show citation

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Mishra, R.K. (2010). Simple method for isolation of oxygen evolving photosystem 2 core complex free of cytochrome f contamination. Biologia plantarum, 54(3), 520-524. doi: 10.1007/s10535-010-0091-2
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