biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:543-546, 2010 | DOI: 10.1007/s10535-010-0096-x

Study of the origin of the rarely cultivated edible Solanum species: morphological and molecular data

P. Poczai1,*, K. Mátyás1, J. Taller1, I. Szabó1
1 Department of Plant Science and Biotechnology, Georgikon Faculty, University of Pannonia, Keszthely, Hungary

The present study applies RAPD technique and morphometric analysis to study the diversity of some accessions belonging to section Solanum. A total of 252 products were amplified with 23 12-mer arbitrary primer pairs, among which 210 were found to be polymorphic. Sixteen morphological characters were measured and used to compile a dendrogram. Both the morphological and RAPD marker analysis clearly separated the different accessions into similar groups. The results indicate that the analyzed cultivars with unknown origin could be derived from S. retroflexum. We found morphological differences among the S. scabrum subsp. scabrum accession which were not reflected in the molecular data. Presumably these accessions represent cultivated forms selected for their habit, fruit quantity and/or quality and leaf size, respectively.

Keywords: genetic diversity; RAPD; Solanum retroflexum; Solanum scabrum
Subjects: growth and growth analysis; potato; random amplified polymorphic DNA (RAPD); Solanum nigrum; Solanum scabrum; Solanum tuberosum; Solanum villosum; UPGMA dengrogram

Received: November 3, 2008; Accepted: June 2, 2009; Published: September 1, 2010  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Poczai, P., Mátyás, K., Taller, J., & Szabó, I. (2010). Study of the origin of the rarely cultivated edible Solanum species: morphological and molecular data. Biologia plantarum, 54(3), 543-546. doi: 10.1007/s10535-010-0096-x
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Cordeiro, A.I., Sanchez-Sevilla, J.F., Alvarez-Tinaut, M.C., Gomez-Jimenez, M.C.: Genetic diversity assessment in Portugal accessions of Olea europea by RAPD markers. - Biol. Plant. 52: 642-647, 2008. Go to original source...
    2. Dehmer, K.J.: Conclusions on the taxonomy of the Solanum nigrum complex by molecular analysis of IPK germplasm accessions. - In: Van de Berg, R.G., Barendse, G.W.M., Van der Weerden, G.M., Mariani, C. (ed.): Solanaceae V: Advances in Taxonomy and Utilization. Pp. 85-96. Nijmegen University Press, Nijmegen, 2001.
    3. Dehmer, K.J., Hammer, K.: Taxonomic status and geographical provenance of germplasm accessions in the Solanum nigrum L. complex: AFLP data. - Genet. Res. Crop Evol. 51: 551-558, 2004. Go to original source...
    4. Dice, L.R.: Measuring of amount of ecological association between species. - Ecology 26: 297-302, 1945. Go to original source...
    5. Edmonds, J.M.: A synopsis of the taxonomy of Solanum sect. Solanum (Maurella) in South America. - Kew. Bull. 27: 79-114, 1972. Go to original source...
    6. Edmonds, J.M., Chweya, J.A. (ed.): Black Nightshades Solanum nigrum L. and Related Species. Promoting the Conservation and Use of Underutilized and Neglected Crops. - Institute of Plant Genetics and Crop Plant Research, Gatersleben and International Plant Genetic Resources Institute, Rome 1997.
    7. Ganapathi, A., Rao, G.R.: The crossability and genetic relationship between Solanum retroflexum Dun. and S. nigrum L. - Cytologia 51: 757-62, 1986. Go to original source...
    8. Lester, R.N. Daunay, M.-C.: Diversity of African vegetable Solanum and its implications for a better understanding of plant domestication. - In: Knüpffer, H., Ochsmann, J. (ed.): Rudolf Mansfeld and Plant Genetic Resources. Proceedings of a Symposium Dedicated to the 100th Birthday of Rudolf Mansfeld. Pp. 137-152, ZADI, Bonn 2003.
    9. Manoko, M.L.K., Van den Berg, R.G., Feron, R.M.C., Van der Weerden, G.M., Mariani, C.: Genetic diversity of the African hexaploid species Solanum scabrum Mill. and Solanum nigrum L. (Solanaceae). - Genet. Res. Crop Evol. 55: 409-418, 2008. Go to original source...
    10. Miller, J.T., Spooner, D.M.: Collapse of species boundaries in the wild potato Solanum brevicaule complex (Solanaceae sect. Petota): molecular data. - Plant. Syst. Evol. 214: 103-130, 1999. Go to original source...
    11. Nei, M., Li, W.H.: Mathematical model for studying genetic variation in terms of restriction endonucleases. - Proc. nat. Acad. Sci. USA 76: 5269-5273, 1979. Go to original source...
    12. Olet, E.A., Huen, M., Lye, K.A.: African crop or poisonous nightshade; the enigma of poisonous or edible black nightshades solved. - Afr. J. Ecol. 43: 158-161, 2005. Go to original source...
    13. Olet, E.A., Heun, M, Lye, K.A.: A new subspecies of Solanum scabrum Miller Found in Uganda. - Novon 16: 508-511, 2006. Go to original source...
    14. Perrier, X., Flori, A., Bonnot, F.: Data analysis methods. - In: Hamon, P., Seguin, M., Perrier, X., Glaszmann, J.C. (ed.): Genetic Diversity of Cultivated Tropical Plants. Pp. 43-76. Enfield, Science Publishers, Montpellier 2003.
    15. Perrier, X., Jacquemoud-Collet, J.P.: DARwin software http://darwin.cirad.fr/darwin, 2006.
    16. Poczai, P., Taller, J., Szabó, I.: Analysis of phylogenetic relationships in the genus Solanum (Solanaceae) as revaled by RAPD markers. - Plant. Syst. Evol. 275: 59-67, 2008. Go to original source...
    17. Ray Choudhury, P., Singh, I.P., George, B., Verma, A.K., Singh, N.P.: Assessment of genetic diversity of pigeonpea cultivars using RAPD analysis. - Biol. Plant. 52: 648-653, 2008. Go to original source...
    18. Refoufi, A., Esnault, M.A.: Population genetic diversity in the polyploid complex of the wheatgrasses using isoenzyme and RAPD data. - Biol. Plant. 52: 543-547, 2008. Go to original source...
    19. Schols, P., D'hondt, C., Geuten, K., Merckx, V., Janssens, S., Smets, E.: MorphoCode: coding quantitative data for phylogenetic analysis. - Phyloinformatics 4:1-4, 2004.
    20. Spooner, D.M., Ugarte, M.L., Skroch, P.W.: Species boundaries and interrelationships of two closely related sympatric diploid wild potato species, Solanum astleyi and S. boliviense, based on RAPDs. - Theor. appl. Genet. 95: 764-771, 1997. Go to original source...
    21. Thiele, K.: The holy grail of the perfect character: the cladistic treatment of morphometric data. - Cladistics 9: 275-304, 1993. Go to original source...
    22. Van de Peer, Y., De Wachter, Y.: TREECON for Windows: a software package for the construction and drawing of evolutionary trees for the Microsoft Windows environments. - Commun. appl. Biosci. 10:569-570, 1994. Go to original source...
    23. Walbot, V., Warren, C.: Regulation of Mu element copy number in maize lines with an active or inactive Mutator transposable element system. - Mol. gen. Genet. 211: 27-34, 1988. Go to original source...
    24. Welsh, J., McClelland, M.: Fingerprinting genomes using PCR with arbitrary primers. - Nucl. Acids Res. 18: 7213-7218, 1990. Go to original source...
    25. Williams, J.G.K, Hanafey, M.K, Rafalski, J.A., Tingey, S.V.: Genetic analysis using random amplified polymorphic DNA markers. - Methods Enzymol. 218: 704-740, 1993. Go to original source...
    26. Willams, J.K.F., Kubelik, A.R., Livak, K.G., Rafalski, J.A., Tingey, S.V.: DNA polymorphism amplified by arbitrary primers are useful as genetic markers. - Nucleic. Acids. Res. 18, 6531-6535, 1990. Go to original source...
    27. Yang, R.W., Zhou, Y.H., Ding, C.B., Zheng, Y.L., Zhang, L.: Relationships among Leymus species assessed by RAPD markers. - Biol. Plant. 52: 237-241, 2008. Go to original source...

    Return to the content