biologia plantarum

Biologia plantarum 54:561-565, 2010 | DOI: 10.1007/s10535-010-0100-5

ISSR marker based analysis of micropropagated plantlets of Nothapodytes foetida

M. Chandrika¹, V. Ravishankar Rai^1,*, Thoyajaksha¹

¹ Department of studies in Applied Botany and Biotechnology, University of Mysore, Mysore, India

A DNA-based, inter simple sequence repeat (ISSR) markers were used to monitor genetic stability in micropropagated plantlets of Nothapodytes foetida. A total of 146 clear and distinct bands were produced using 26 primers resulting in 3 212 fragments. Out of 146, 135 bands (92.4 %) were monomorphic and 11 bands (7.53 %) were polymorphic which ranged from 200 to 21 226 bp in size. The number of bands per each primer varied from 1 to 11 with an average of 5.6 bands per primer. The banding pattern for each primer was uniform and comparable to mother plant from which the cultures had been established. The dendrogram based on the unweighted pair-group method with arithmetic averaging (UPGMA) depicted about 97 % homology between the mother plant and micropropagated plants. An attempt was made to reintroduce the micropropagated plants in the natural habitat and over 500 plants were successfully established.

Keywords: genetic stability; medicinal tree; PCR; reintroduction; UPGMA

Subjects: ex vitro transfer; genetic stability; inter-simple sequence repeat (ISSR); Nothapodytes foetida; polymerase chain reaction (PCR); UPGMA dengrogram

Received: November 14, 2008; Accepted: May 20, 2009; Published: September 1, 2010  Show citation