biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 54:587-591, 2010 | DOI: 10.1007/s10535-010-0106-z

Analysis of Lupinus albus heat-shock granule proteins in response to high temperature stress

S. Cherian1,*, R. B. Ferreira1,2
1 Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Oeiras, Portugal
2 Departmento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Lisboa, Portugal

An important aspect of heat-shock response of lupin (Lupinus albus cv. Rio Maior) is the formation of cytoplasmic granular aggregates, called heat-shock granules (HSGs). In this study, two-dimensional electrophoresis (2-DE) was used to detect the component proteins of HSG complexes formed in vivo. Evaluation of 2-DE revealed differential expression of several proteins under heat shock conditions when compared with control. Among them, small heat-shock proteins (sHSPs) of 15 to 30 kDa were found to be the major representative proteins along with other proteins of relative molecular mass ranging from 36 to 45 kDa and above.

Keywords: molecular chaperone; small heat-shock proteins; thermotolerance
Subjects: heat shock proteins (HSP); lupin; Lupinus albus; proteins; temperature high

Received: December 6, 2008; Accepted: May 17, 2009; Published: September 1, 2010  Show citation

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Cherian, S., & Ferreira, R.B. (2010). Analysis of Lupinus albus heat-shock granule proteins in response to high temperature stress. Biologia plantarum, 54(3), 587-591. doi: 10.1007/s10535-010-0106-z
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