biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:703-706, 2010 | DOI: 10.1007/s10535-010-0124-x

Microwave treatment induced mutations and altered gene expression in Vigna aconitifolia

R. K. Jangid1, R. Sharma1, Y. Sudarsan1,*, S. Eapen3, G. Singh1, A. K. Purohit2
1 Plant Biotechnology Centre, Rajasthan Agricultural University, Bikaner, India
2 ASC-DEC, Rajasthan Agricultural University, Bikaner, India
3 NABTD, Bhabha Atomic Research Centre, Trombay, Mumbai, India

Primary leaf explants of aseptically grown seedlings of moth bean [Vigna aconitifolia (Jacq.) Marechal] immersed in water or not were treated in microwave oven (2450 MHz, 800 W cm-2) for 1, 3, 5 and 7 s before culturing. Callusing and shoot emergence from these explants were enhanced up to microwave exposure lasted 5 s while longer treatment of water-immersed explants delayed callusing. One polypeptide (26.6 kD) was up regulated in the callus derived from microwave treatment in water-immersed explants. RAPD analysis detected alteration in DNA sequences due to microwave treatment in water-immersed explants for 7 s. The frequency of mutation was 1.6 % (4 bands out of 248) over all the cultures analyzed and the same was 13 % (4 bands out of 31), if amplicons generated at 7 s treatment alone were considered.

Keywords: in vitro culture; moth bean; RAPD
Subjects: in vitro culture, callus; microwave treatment; mont bean; mutagenesis, mutants; proteins; random amplified polymorphic DNA (RAPD); Vigna aconitifolia

Received: July 29, 2008; Accepted: June 11, 2009; Published: December 1, 2010  Show citation

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Jangid, R.K., Sharma, R., Sudarsan, Y., Eapen, S., Singh, G., & Purohit, A.K. (2010). Microwave treatment induced mutations and altered gene expression in Vigna aconitifolia. Biologia plantarum, 54(4), 703-706. doi: 10.1007/s10535-010-0124-x
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