Biologia plantarum 54:707-710, 2010 | DOI: 10.1007/s10535-010-0125-9
Isolation and expression of gene encoding leucoanthocyanidin reductase from Diospyros kaki during fruit development
- 1 Department of Pomology, Key Laboratory for Horticultural Plant Biology, Huazhong Agricultural University, Wuhan, P.R. China
Leucoanthocyanidin reductase (LAR) converts leucoanthocyanidin to (+)-catechin, a precursor of proanthocyanidins abundant in Japanese persimmon (Diospyros kaki Thunb.) fruits. A putative LAR gene (DkLAR) was isolated by rapid amplification of cDNA ends from young fruits. The full-length cDNA of DkLAR gene was 1 356 bp long and encoded an open reading frame of 349 residues. The deduced DkLAR protein was closely related to the homolog in other plant species. The expression of the DkLAR gene in Chinese pollination-constant non-astringent (PCNA) genotype was coincident with the tannin cell development, but was not in Japanese PCNA and Chinese pollination-variant astringent (PCA) genotypes.
Keywords: condensed tannin; Japanese persimmon; real-time RT-PCR
Subjects: Agrobacterium tumefaciens; Diospyros kaki; insect resistance; leucoanthocyanidin reductase; Northern blot analyses; persimmon; Pinus armandii; polymerase chain reaction (PCR); proteins; rapid amplification of cDNA ends (RACE); Southern blot analysis; tannins; transgenic plants; UPGMA dengrogram
Received: December 17, 2008; Accepted: June 18, 2009; Published: December 1, 2010 Show citation
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Wang, Y., Zhang, Q.L., & Luo, Z.R. (2010). Isolation and expression of gene encoding leucoanthocyanidin reductase from Diospyros kaki during fruit development. Biologia plantarum, 54(4), 707-710. doi: 10.1007/s10535-010-0125-9
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