biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 54:757-760, 2010 | DOI: 10.1007/s10535-010-0136-6

In vitro regeneration of Solanum nigrum with enhanced solasodine production

M. A. Bhat, A. Mujib*, A. Junaid, Mahmooduzzafar
Department of Botany, Faculty of Science, Hamdard University, New Delhi, India

High frequency of shoot formation was achieved from Solanum nigrum L. leaves on Murashige and Skoog (MS) medium without any callusing stage. Shoot forming ability was more pronounced on leaves positioned dorsally. For shoot induction, 2.0 mg dm-3 benzylaminopurine and 1.5 mg dm-3 kinetin were observed to be the most effective plant growth regulators (PGRs). The present paper also describes first successful induction of in vitro flowering in S. nigrum. The leaf derived shoots were excised and treated with various root promoting PGRs and 0.25 mg dm-3 indole-3-butyric acid produced maximum number of roots (15.2 per plant). Plants were later transplanted in field with 100 % survival. Solasodine content was higher in in vitro raised shoots and leaf derived callus, compared to ex vitro grown shoots.

Keywords: black nightshade; HPTLC; in vitro flowering; plant growth regulators
Subjects: amino acids; auxins; black nightshade; chlorophyll a,b; cytokinins; in vitro culture, direct organogenesis; in vitro culture, rooting; proteins; Solanum nigrum; sugars

Received: May 9, 2009; Accepted: January 4, 2010; Published: December 1, 2010  Show citation

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Bhat, M.A., Mujib, A., Junaid, A., & Mahmooduzzafar, (2010). In vitro regeneration of Solanum nigrum with enhanced solasodine production. Biologia plantarum, 54(4), 757-760. doi: 10.1007/s10535-010-0136-6
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