biologia plantarum

Biologia plantarum 55:112-118, 2011 | DOI: 10.1007/s10535-011-0015-9

Effects of jasmonate and some other signalling factors on bean and onion growth during the initial phase of cadmium action

W. Maksymiec^1,*

¹ Department of Plant Physiology, Maria Curie-Skłodowska University, Lublin, Poland

Short-time direct and indirect effects of 25 μM Cd on the growth of dicotyledon (Phaseolus coccineus) and monocotyledon (Allium cepa) plants were investigated in the presence of inhibitors of ethylene synthesis, NADPH oxidase, and the octadecanoid pathway. Only 5 min-long action of Cd was enough for inhibition of growth in bean roots, but its recovery time was extended to several days. After 7 h treatment, Cd was significantly accumulated in bean roots, but maximum H₂O₂ accumulation was seen after 1 h. Cd-induced H₂O₂ accumulation decreased especially after addition of ethylene inhibitor silver thiosulphate (STS). Low Cd accumulation and high growth inhibition were observed also in bean leaves and in A. cepa roots. The inhibitors of the octadecanoid pathway greatly weakened the inhibitory effect of Cd in P. coccineus roots, while no significant effect was observed in A. cepa. NADPH oxidase and ethylene blockade reversed (in the case of bean plants and indirectly treated A. cepa plants) or significantly diminished Cd action. Cd-induced growth inhibition of P. coccineus leaves was also alleviated by most inhibitors of the jasmonate pathway and by STS. These results indicate that Cd may have indirect and direct effects on growth processes.

Keywords: Allium cepa; ethylene; heavy metals; hydrogen peroxide; NADPH oxidase; Phaseolus coccineus; stress

Received: May 8, 2009; Accepted: October 30, 2009; Published: March 1, 2011  Show citation