biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:119-124, 2011 | DOI: 10.1007/s10535-011-0016-8

Characterization of a type 3 metallothionein isolated from Porteresia coarctata

B. Usha1, N. S. Keeran1, M. Harikrishnan1, K. Kavitha1, A. Parida1,*
1 Plant Molecular Biology Laboratory, M.S. Swaminathan Research Foundation, Taramani, Chennai, India

Metallothioneins are involved in detoxification of heavy metals. A cDNA encoding type 3 metallothionein (PcMT3) was isolated from the salt stressed leaf cDNA library of Porteresia coarctata (Roxb.) Tateoka (wild rice) that grows well in the heavy metal laden estuarine soils. The PcMT3 cDNA (581 bp) encodes a protein of 64 amino acids. PcMT3 is highly homologous (82 %) to OsMT-I-3a of rice, but is unique from other type 3 plant MTs due to the presence of an additional glycine residue in the C-terminal domain. Analysis of the 5' upstream region of PcMT3 showed the presence of cis-acting elements like the CG box and STRE previously reported to be involved in gene expression under heavy metal stress. Southern analysis suggested the presence of more than one copy of PcMT3-like sequences in the P. coarctata genome. Analysis of genomic clone of PcMT3 revealed the presence of two introns. A comparison of the genomic sequence of PcMT3 with closely similar type 3 MTs from rice and mangrove species revealed conservation in the number and position of introns. Transcript profiling for PcMT3 in P. coarctata leaves in the presence of Cd, Cu and Zn showed an increase in transcript accumulation.

Keywords: cis-acting elements; heavy metals; salt stress; wild rice

Received: January 21, 2009; Accepted: October 20, 2009; Published: March 1, 2011  Show citation

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Usha, B., Keeran, N.S., Harikrishnan, M., Kavitha, K., & Parida, A. (2011). Characterization of a type 3 metallothionein isolated from Porteresia coarctata. Biologia plantarum, 55(1), 119-124. doi: 10.1007/s10535-011-0016-8
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    References

    1. Abdullah, S.N.A., Cheah, S.C., Murphy, D.J.: Isolation and characterization of two divergent type 3 metallothioneins from oil palm, Elaeis guineenis. - Plant Physiol. Biochem. 40: 255-263, 2002. Go to original source...
    2. Akashi, K., Nishimura N., Ishida, Y., Yokota, A.: Potent hydroxyl radical scavenging activity of drought-induced type-2 metallothionein in wild watermelon. - Biochem. biophys. Res. Commun. 323: 72-78, 2004. Go to original source...
    3. Altschul, S.F., Gish, W., Miller, W., Myers, E.W., Lipman, D.J.: Basic local alignment search tool. - J. mol. Biol. 215: 403-410, 1990. Go to original source...
    4. Bačkor, M., Loppi, S.: Interactions of lichens with heavy metals. - Biol. Plant. 53: 214-222, 2009. Go to original source...
    5. Chen, H.J., Hou, W.C., Yang, C.Y., Huang, D.J., Liu, J.S., Lin, Y.H.: Molecular cloning of two metallothionein-like protein genes with differential expression patterns from sweet potato (Ipomoea batatas) leaves. - J. Plant Physiol. 160: 547-555, 2003. Go to original source...
    6. Cobbett, C., Goldsbrough, P.: Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. - Annu. Rev. Plant Biol. 53: 159-182, 2002. Go to original source...
    7. Coyle, P., Philcox, J.C., Carey, L.C., Rofe, A.M.: Metallothionein: the multi purpose protein. - Cell. Mol. Life Sci. 59: 627-47, 2002. Go to original source...
    8. Dellaporta, S.L., Wood, V.P., Hicks, J.B.: A plant DNA minipreparation: version II. - Plant mol. Biol. Rep. 1: 19-21, 1983. Go to original source...
    9. Elmayan, T., Tepfer, M.L.: Evaluation in tobacco of the organ specificity and strength of the rolD promoter, domain A of the 35S promoter and the 35S2 promoter. - Transgenic Res. 4: 388-396, 1995. Go to original source...
    10. Evans, I.M., Gatehouse, J.N., Gatehouse, J.A., Robinson, N.J., Croy R.R.D.: A gene from pea (Pisum sativum L) with homology to metallothionein genes. - FEBS Lett. 262: 29-32, 1990. Go to original source...
    11. Foley, R.C., Liang, Z.M., Singh, K.B.: Analysis of type 1 metallothionein cDNAs in Vicia faba. - Plant mol. Biol. 33: 583-591, 1997. Go to original source...
    12. Fordham-Skelton, A.P., Lilley, C., Urwin, P.E., Robinson, N.J.: GUS expression in Arabidopsis directed by 5' regions of the pea metallothionein gene PsMTA. - Plant mol. Biol. 34: 659-668, 1997. Go to original source...
    13. Garcia-Hernandez, M., Murphy, A., Taiz, L.: Metallothioneins 1 and 2 have distinct but overlapping expression patterns in Arabidopsis. - Plant Physiol. 118: 387-397, 1998. Go to original source...
    14. Gisela, M, Jordi, D., Gemma, H., Guo, W.J., Goldsbrough, P., Atrian, S., Molinas, M.: A plant type 2 metallothionein (MT) from cork tissue responds to oxidative stress. - J. exp. Bot. 55: 2483-2493, 2004. Go to original source...
    15. Gonzalez-Mendoza, D., Moreno, A.Q., Zapata-Perez, O.: Coordinated responses of phytochelatin synthase and metallothionein genes in black mangrove, Avicennia germinans, exposed to cadmium and copper. - Aquat. Toxicol. 83: 306-314, 2007. Go to original source...
    16. Guo, W.J., Bundithya, W., Goldsbrough, P.B.: Characterization of the Arabidopsis metallothionein gene family: tissuespecific expression and induction during senescence and in response to copper. - New Phytol. 159: 369-381, 2003. Go to original source...
    17. Haq, F., Mahoney, M., Koropatnick, J.: Signalling events for metallothionein induction. - Mutat. Res. 523: 211-226, 2003. Go to original source...
    18. Hsieh, H.M., Huang, P.C.: Promoter structure and activity of type 1 rice metallothionein-like gene. - DNA Sequence 9: 9-17, 1998. Go to original source...
    19. Higo, K., Ugawa, Y., Iwamoto, M., Korenaga, T.: Plant cisacting regulatory DNA element (PLACE) database. - Nucl. Acids Res. 27: 297-300, 1999. Go to original source...
    20. Jagtap, T.G., Bhosale, S., Charulata, S.: Characterization of Porteresia coarctata beds along the Goa coast, India. - Aquat. Bot. 84: 37-44, 2006. Go to original source...
    21. Kohler, A., Blaudez, D., Chalot, M., Martin, F.: Cloning and expression of multiple metallothioneins from hybrid poplar. - New Phytol. 164: 83-93, 2004. Go to original source...
    22. Latha, R.: Studies on propagation, genetic relationship and characterization of salinity tolerance in P. coarctata (Roxb.) Tateoka, a wild relative of rice. - Ph.D. Thesis. University of Madras, Madras 2000.
    23. Lee, J., Shim, D., Song, W.Y., Hwang, I., Lee, Y.: Arabidopsis metallothioneins 2a and 3 enhance resistance to cadmium when exposed in Vicia faba guard cells. - Plant mol. Biol. 54: 805-815, 2004. Go to original source...
    24. Liu, Y.G., Mitsukawa, N., Oosumi, T., Whittier, R.F.: Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR. - Plant J. 8: 457-463, 1995. Go to original source...
    25. Murphy, A., Zhou, J., Goldsbrough P., Taiz, L.: Purification and immunological identification of metallothioneins 1 and 2 from Arabidopsis thaliana. - Plant Physiol. 113: 1293-1301, 1997. Go to original source...
    26. Navabpour, S., Morris, K., Allen, R., Harrison, E., Mackerness, S., Buchanan-Wollaston, Y.: Expression of senescenceenhanced genes in response to oxidative stress. - J. exp. Bot. 54: 2285-2292, 2003. Go to original source...
    27. Robinson, N.J., Tommey, A.M., Kuske, C., Jackson, P.J.: Plant metallothioneins. - Biochem. J. 295: 1-10, 1993. Go to original source...
    28. Roosens, N.H., Leplae, R., Bernard, C., Verbruggen, N.: Variations in plant metallothioneins: the heavy metal hyperaccumulator Thlaspi caerulescens as a study case. - Planta 222: 716-729, 2005. Go to original source...
    29. Ruis, H., Koller, F.: Biochemistry, molecular biology, and cell biology of yeast and fungal catalases. - In: Scandalios, J.G. (ed.): Oxidative Stress and Molecular Biology of Antioxidant Defenses. Pp. 309-342. Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1997.
    30. Sambrook, T., Fritsch, E.F., Maniatis, T.: Molecular Cloning: a Laboratory Manual. - Cold Spring Harbor Laboratory Press, Cold Spring Harbor - New York 1989.
    31. Senthilkumar, P., Jithesh, M.N., Parani, M., Rajalakshmi, S., Praseetha, K., Parida, A.: Salt stress effects on the accumulation of vacuolar H+-ATPase subunit C transcripts in wild rice, Porteresia coarctata (Roxb.) Tateoka. - Curr. Sci. 89: 1386-1394, 2005.
    32. Sessions, A., Burke, E., Presting, G., Aux, G., McElver, J., Patton, D., Dietrich, B., Ho, P., Bacwaden, J., Ko, C., Clarke, J.D., Cotton, D., Bullis, D., Snell, J., Miguel, T., Hutchison, D., Kimmerly, B., Mitzel, T., Katagiri, F., Glazebrook, J., Law, M., Goff, S.A.: A highthroughput Arabidopsis reverse genetics system. - Plant Cell 14: 2985-2994, 2002. Go to original source...
    33. Shanker, A.K., Djanaguiraman, M., Sudhagar, R., Jayaram, K., Pathmanaban, G.: Expression of metallothionein 3-like protein mRNA in sorghum under chromium (VI) stress. - Curr. Sci. 86: 901-902, 2004.
    34. Snowden, K.C., Buchholz, W.G., Hall, T.C.: Intron position affects expression from the tpi promoter in rice. - Plant mol. Biol. 31: 689-692, 1996. Go to original source...
    35. Tam, N.F.Y., Wong, Y.S.: Retention of nutrients and heavy metals in mangrove sediment receiving wastewater of different strengths. - Environ. Technol. 14: 719-729, 1993. Go to original source...
    36. Thompson, J.D., Higgins, D.G., Gibson, T.J.: CLUSTALW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. - Nucl. Acids Res. 11: 4673-4680, 1994. Go to original source...
    37. Usha, B., Prashanth, S.R., Parida, A.: Differential expression of two metallothionein encoding genes during heavy metal stress in the mangrove species, Avicennai marina (Forsk.) Vierh. - Curr. Sci. 93: 1215-1219, 2007.
    38. Usha, B., Venkataraman, G., Parida, A.: Heavy metal and abiotic stress inducible metallothionein isoforms from Prosopis juliflora (SW) D.C. show differences in binding to heavy metals in vitro. - Mol. Genet. Genom. 281: 99-108, 2009. Go to original source...
    39. Whitelaw, C.A., Huquet, J.A.L., Thurman, D.A., Timsett, A.B.: The isolation and characterization of type II metallothionein-like genes from tomato (Lycopersicum esculentum L). - Plant mol. Biol. 33: 503-511, 1997. Go to original source...
    40. Wong, H.L., Sakamoto, T., Kawasaki, T., Umemura, K., Shimamoto, K.: Down regulation of metallothionein, a reactive oxygen scavenger, by the small GTPase OsRac1 in rice. - Plant Physiol. 135: 1447-1456, 2004. Go to original source...
    41. Yang, T., Poovaiah, R.W.: A calmodulin-binding/CGCG box DNA-binding protein family involved in multiple signaling pathways in plants. - J. biol. Chem. 277: 45049-45058, 2002. Go to original source...
    42. Yu, L.H., Liu, J.Y., Umeda, M., Uchimia, H.E.: Molecular characterization of metallothionein genes in plants. - Plant Biotechnol. 15: 167-172, 1998. Go to original source...
    43. Zhou, J., Goldsbrough, P.R.: Functional homologs of fungal metallothionein genes from Arabidopsis. - Plant Cell 6: 875-884, 1994. Go to original source...
    44. Zhou, J., Goldsbrough, PR.: Structure organization and expression of the metallothionein gene family in Arabidopsis. - Mol. gen. Genet. 248: 318-328, 1995. Go to original source...
    45. Zhou, G.K., Xu, Y.F., Li, J., Yang, L., Liu, J.Y.: Molecular analysis of the metallothionein gene family in rice (Oryza sativa L.). - J. Biochem. mol. Biol. 39: 595-606, 2006. Go to original source...
    46. Zhou, G.K., Xu, Y.E., Liu, J.Y.: Characterization of a rice class II metallothionein gene: tissue expression patterns and induction in response to abiotic factors. - J. Plant Physiol. 162: 686-696, 2005. Go to original source...

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