biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:169-172, 2011 | DOI: 10.1007/s10535-011-0024-8

In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora

P. Bantawa1, O. Saha-Roy1, S. Kumar Ghosh1, T. Kumar Mondal1,*
1 Biotechnology Laboratory, Faculty of Horticulture, Uttar Banga Krishi Viswavidalaya, Pundibari, Cooch Behar, India

A reproducible in vitro regeneration system for Nepalese kutki (Picrorhiza scrophulariiflora Pennell) was developed from in vitro leaf derived callus. Induction of more than seven shoot buds per explant was achieved on Woody plant medium (WPM) supplemented with 0.53 μM α-napthaleneacetic acid (NAA) and 0.23 μM kinetin (KIN). The shoots were elongated on WPM supplemented with 0.44 μM 6-benzylaminopurine (BAP) and rooted on WPM supplemented with 5.3 μM NAA within 2 weeks. The random amplified polymorphic DNA (RAPD) analysis indicated genetic uniformity of the micropropagated plants with its donor plants.

Keywords: auxins; callus induction; cytokinins; picroside

Received: May 7, 2009; Accepted: November 15, 2009; Published: March 1, 2011  Show citation

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Bantawa, P., Saha-Roy, O., Kumar Ghosh, S., & Kumar Mondal, T. (2011). In vitro regeneration of an endangered medicinal plant Picrorhiza scrophulariiflora. Biologia plantarum, 55(1), 169-172. doi: 10.1007/s10535-011-0024-8
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