biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:331-334, 2011 | DOI: 10.1007/s10535-011-0048-0

Overexpression of a Ran GTPase homologous gene, FaRan from tall fescue, in transgenic Arabidopsis

S. H. Lü1,*, Y. L. Fan1, C. X. Jin2
1 School of Agriculture, Liaocheng University, Liaocheng, P.R. China
2 School of Chemistry and Environmental Sciences, Henan Normal University, Henan, P.R. China

We isolated and characterized a novel Ran GTPase homologous gene, FaRan from tall fescue (Festuca arundinacea Schreb.). Reverse transcriptase polymerase chain reaction (RT-PCR) analysis indicated that FaRan is broadly expressed in old mature leaves, young leaves, plumules, stems, infloresence meristems, but at different levels. Transcript of FaRan is higher in young meristems than in old ones. Ectopic expression of FaRan resulted in increased number of axillary buds and reduced apical dominance in transgenic Arabidopsis plants. These results suggest that FaRan in F. arundinacea may be involved in the initiation of meristem and subsequent growth as well as development. In addition, it also suggests that FaRan can be used potentially to improve turfgrass quality.

Keywords: Festuca arundinacea; growth and development; RT-PCR

Received: May 29, 2009; Accepted: January 15, 2010; Published: June 1, 2011  Show citation

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Lü, S.H., Fan, Y.L., & Jin, C.X. (2011). Overexpression of a Ran GTPase homologous gene, FaRan from tall fescue, in transgenic Arabidopsis. Biologia plantarum, 55(2), 331-334. doi: 10.1007/s10535-011-0048-0
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