biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:349-352, 2011 | DOI: 10.1007/s10535-011-0052-4

Expression of a yeast polygalacturonase gene in Arabidopsis thaliana

M. Prieto-Alcedo1, P. Veiga-Crespo1, M. Poza1, C. Coronado3, I. Zarra2, T. G. Villa1,*
1 Facultad de Farmacia, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
2 Facultad de Biología, Universidad de Santiago de Compostela, Santiago de Compostela, Spain
3 Escuela Universitaria de Enfermería y Podología, Universidad de A Coruña, Ferrol, Spain

Polygalacturonases are enzymes involved in plant cell wall growth and reorganization. Transgenic Arabidopsis thaliana plants with a Saccharomyces cerevisiae endopolygalacturonase gene (PGU1) were obtained. The yeast gene was properly expressed in the plants as it has been shown by RT-PCR as well as by the increase in the endopolygalacturonase activity. The transgenic plants showed conspicuous malformations in early stages of development probably due to a weak cell adhesion. On the other hand, adult plants exhibited almost no phenotypic differences as compared to the wild type plants, this suggesting the appearance of some mechanisms on the plant side to counteract the effect of the overexpressed polygalacturonase.

Keywords: cell wall; RT-PCR; Saccharomyces cerevisiae; transgenic plants

Received: July 31, 2009; Accepted: February 27, 2010; Published: June 1, 2011  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Prieto-Alcedo, M., Veiga-Crespo, P., Poza, M., Coronado, C., Zarra, I., & Villa, T.G. (2011). Expression of a yeast polygalacturonase gene in Arabidopsis thaliana. Biologia plantarum, 55(2), 349-352. doi: 10.1007/s10535-011-0052-4
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Atkinson, R., Schröder, R., Hallett, I.C., Cohen, D., MacRae, E.A.: Overexpression of polygalacturonase in transgenic apple trees leads to a range of novel phenotypes involving changes in cell adhesion. - Plant Physiol. 129: 122-133, 2002. Go to original source...
    2. Bevan, M.W.: Binary Agrobacterium vectors for plant transformation. - Nucl. Acids Res. 12: 8711-8721, 1984. Go to original source...
    3. Blanco, P., Sieiro, C., Reboredo, N.M., Villa, T.G.: Cloning, molecular characterization, and expression of an endopolygalacturonase-encoding gene from Saccharomyces cerevisiae IM1-8b. - FEMS Lett. 164: 249-255, 1998. Go to original source...
    4. Bonghi, C., Rascio, N., Ramina, A., Casadoro, G.: Cellulase and polygalacturonase involvement in the abscission of leaf and fruit explants of peach. - Plant mol. Biol. 20: 839-848, 1992. Go to original source...
    5. Capodicasa, C., Vairo, D., Zabotina, O., McCartney, L., Caprari, C., Mattei, B., Manfredini, C., Aracri, B., Benen, J., Knox, J.P., De Lorenzo, G., Cervone, F.: Targeted modification of homogalacturonan by transgenic expression of a fungal polygalacturonase alters plant growth. - Plant Physiol. 135: 1294-1304, 2004. Go to original source...
    6. Carpita, N.C., Gibeaut, D.M.: Structural models of primary cell walls in flowering plants: consistency of molecular structure with the physical properties of the walls during growth. - Plant J. 3: 1-30, 1993. Go to original source...
    7. Clough, J.S., Bent, F.A.: Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana. - Plant J. 16: 735-743, 1998. Go to original source...
    8. Di Matteo, A., Bonivento, D., Tsernoglou, D., Federici, L., Cervone, F.: Polygalacturonase-inhibiting protein (PGIP) in plant defence: a structural view. - Phytochemistry 67: 528-533, 2006. Go to original source...
    9. D'Ovidio, R., Mattei, B., Roberti, S., Bellincampi, D.: Polygalacturonases, polygalacturonase-inhibiting proteins and pectic oligomers in plant-pathogen interactions. - Biochem. biophys. Acta 1696: 237-244, 2004. Go to original source...
    10. Fry, S.C.: Primary cell wall metabolism: tracking the careers of wall polymers in living plant cells. - New Phytol. 161: 641-675, 2004. Go to original source...
    11. Hood, E.E., Gelvin, S.B., Melcehers, L.S., Hoekema, A.: New Agrobacterium helper plasmids for gene transfer to plants. - Transgenic Res. 2: 208-218, 1993. Go to original source...
    12. Huang, C., Guo, T., Zheng, S.C., Feng, Q.L., Liang, J.H., Li, L.: Increased cold tolerance in Arabidopsis thaliana transformed with Choristoneura fumiferana glutathione Stransferase gene. - Biol. Plant. 53: 183-187, 2009. Go to original source...
    13. Lever, M.: A new reaction for colorimetric determination of carbohydrates. - Anal. Biochem. 47: 273-279, 1972. Go to original source...
    14. O'Neill, M.A., York, W.S.: The composition and structure of plant primary cell walls. - In: Rose, J.K.C. (ed.): The Plant Cell Wall. Pp. 1-54. Blackwell Publishers, Oxford 2003. Go to original source...
    15. Osteryoung, K.W., Toenjes, K., Hall, B.D., Winkler, V., Bennett, A.B.: Analysis of tomato polygalacturonase expression in transgenic tobacco. - Plant Cell. 2: 1239-1248, 1990. Go to original source...
    16. Ridley, B.L., O'Neill, M.A., Mohnen, D.: Pectins: structure, biosynthesis, and oligogalacturonide-related signalling. - Phytochemistry 57: 929-967, 2001. Go to original source...
    17. Solecka, D., Zebrowski, J., Kacperska, A.: Are pectins involved in cold acclimation and de-acclimation of winter oil-seed rape plants? - Ann. Bot. 101: 521-530, 2008. Go to original source...
    18. Somerville, C., Bauer, S., Brininstool, G., Facette, M., Hamann, T., Milne, J., Osborne, E., Paredez, A., Persson, S., Raab, T., Vorwerk, S., Youngs. H.: Toward a systems approach to understanding plant cell walls. - Science 306: 2206-2211, 2004. Go to original source...
    19. Veronesi, C., Bonnin, E., Calvez, S., Thalouarn, P., Simier, P.: Activity of secreted cell wall-modifying enzymes and expression of peroxidase-encoding gene following germination of Orobanche ramose. - Biol. Plant. 51: 391-394, 2007. Go to original source...

    Return to the content