biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:387-390, 2011 | DOI: 10.1007/s10535-011-0061-3

Isolation of 55 microsatellite markers for Jatropha curcas and its closely related species

C. Phumichai1,*, T. Phumichai2, N. Kongsiri1, A. Wongkaew1, P. Sripichit1, R. Kaveeta1
1 Department of Agronomy, Faculty of Agriculture, Kasetsart University, Bangkok, Thailand
2 Department of Agriculture, Rubber Research Institute of Thailand, Bangkok, Thailand

Jatropha curcas L. (physic nut) is native to Central America and now naturalized widely in many tropical and subtropical areas. Microsatellite markers were isolated and characterized. Eleven out of 55 markers showed polymorphisms, and the allelic variation was investigated using 26 accessions of J. curcas collected from several provinces in Thailand. Each marker showed 2 to 5 alleles and the average polymorphic information content (PIC) was 0.49. Thirty four markers (62 %) were also successfully amplified in J. integerrima, J. gossypifolia and J. podagrica.

Keywords: genetic diversity; microsatellite-enriched genomic library; physic nut

Received: November 14, 2009; Accepted: June 8, 2010; Published: June 1, 2011  Show citation

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Phumichai, C., Phumichai, T., Kongsiri, N., Wongkaew, A., Sripichit, P., & Kaveeta, R. (2011). Isolation of 55 microsatellite markers for Jatropha curcas and its closely related species. Biologia plantarum55(2), 387-390. doi: 10.1007/s10535-011-0061-3
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