biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:549-553, 2011 | DOI: 10.1007/s10535-011-0123-6

Extracellular matrix as the early structural marker for Centella asiatica embryogenic tissues

K. S. Lai1, K. Yusoff2, M. Maziah1,*
1 Department of Biochemistry, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor DE, Malaysia
2 Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Selangor DE, Malaysia

Embryogenic and non-embryogenic calli were induced from the Centella asiatica leaf explants on Murashige and Skoog medium supplemented with kinetin and 2,4-dichlorophenophenoxyacetic acid. The extracellular matrix (ECM) layer was seen on the surface of embryogenic cells but not on the non-embryogenic cells. The ECM formed bridges with net-like material between the embryogenic cells. This network like structure was believed to play an important role in plant morphogenesis and can serve as an early structural marker of embryogenic competence in Centella asiatica calli culture.

Keywords: auxin; cytokinin; embryogenic cells; SEM; TEM

Received: October 4, 2009; Accepted: March 16, 2011; Published: September 1, 2011  Show citation

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Lai, K.S., Yusoff, K., & Maziah, M. (2011). Extracellular matrix as the early structural marker for Centella asiatica embryogenic tissues. Biologia plantarum, 55(3), 549-553. doi: 10.1007/s10535-011-0123-6
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