biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:554-558, 2011 | DOI: 10.1007/s10535-011-0124-5

High frequency plant regeneration from the mature seeds of Garcinia indica

M. Baskaran1, S. Krishnan1,*
1 Department of Botany, Goa University, Goa, India

A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured on Woody plant medium (WPM) supplemented with 2.0 mg dm-3 N6-benzyladenine and 1 mg dm-3 indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm-3 indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation from mature seed segments.

Keywords: direct organogenesis; kokum elite clones; mass multiplication; multiple shoots

Received: November 8, 2009; Accepted: March 31, 2010; Published: September 1, 2011  Show citation

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Baskaran, M., & Krishnan, S. (2011). High frequency plant regeneration from the mature seeds of Garcinia indica. Biologia plantarum, 55(3), 554-558. doi: 10.1007/s10535-011-0124-5
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