biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:703, 2011 | DOI: 10.1007/s10535-011-0171-y

Callus sieving is effective in improving synchronization and frequency of somatic embryogenesis in Citrus sinensis

J. M. M. Souza1,2, M. L. Tomaz2, S. C. C. Arruda2, C. G. B. Demétrio1, W. N. Venables3, A. P. Martinelli2,*
1 Escola Superior de Agricultura 'Luiz de Queiroz', Universidade de São Paulo, Piracicaba, SP, Brazil
2 Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba, SP, Brazil
3 CSIRO Division of Mathematics and Information Sciences, Cleveland, Australia

Somatic embryogenesis has been obtained in many citrus cultivars. However, culture synchronization is yet to be achieved and in the present work we evaluate the effect of desiccation, cold and size of cell clusters on embryo production efficiency from callus cultures of Citrus sinensis L. Osbeck, cv. Valencia. The results showed that sieving was effective in promoting somatic embryo synchronization, whether or not it was followed by cold or desiccation treatments. Histological and histochemical analyses are presented to characterize the structure of cell aggregates and protein accumulation.

Keywords: cell aggregates; cold; desiccation; histology; proteins

Received: October 5, 2009; Accepted: June 29, 2010; Published: December 1, 2011  Show citation

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Souza, J.M.M., Tomaz, M.L., Arruda, S.C.C., Demétrio, C.G.B., Venables, W.N., & Martinelli, A.P. (2011). Callus sieving is effective in improving synchronization and frequency of somatic embryogenesis in Citrus sinensis. Biologia plantarum, 55(4), 703. doi: 10.1007/s10535-011-0171-y
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