biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 55:761, 2011 | DOI: 10.1007/s10535-011-0183-7

Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping

J. Patzak1,*, J. Matoušek2
1 Hop Research Institute, Co. Ltd., Žatec, Czech Republic
2 Intitute of Plant Molecular Biology, Biology Centre of the Academy of Science of the CR, České Budějovice, Czech Republic

The use of expressed sequence tag-simple sequence repeat (EST-SSR) markers might reflect the better relationship among species or cultivars than markers previously used. The first set of 30 EST-SSR was developed in hop (Humulus lupulus L.). They represent 25 gene loci with total of 1268 EST sequences. They were used for characterization of 11 hop samples and cross-amplification in Humulus japonicus Sieb. et Zucc. The number of alleles per locus ranged from two to nine. The observed and expected heterozygosities ranged from 0.182 to 0.956 and from 0.233 to 0.775, respectively. We used EST-SSR markers for cluster analysis of hop genotypes. Dendrogram well matched with genealogical and geographical data for hop genotypes.

Keywords: cluster analysis; dendrogram; Humulus lupulus; Humulus japonicus; simple sequence repeat

Received: April 12, 2010; Accepted: June 29, 2010; Published: December 1, 2011  Show citation

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Patzak, J., & Matoušek, J. (2011). Development and evaluation of expressed sequence tag-derived microsatellite markers for hop genotyping. Biologia plantarum, 55(4), 761. doi: 10.1007/s10535-011-0183-7
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