biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:140-144, 2012 | DOI: 10.1007/s10535-012-0030-5

Molecular cloning and characterization of nucleoside diphosphate kinase 1 cDNA in tea

G. R. Prabu1,2, K. Thirugnanasambantham1, A. K. A. Mandal1,3,*, A. Saravanan1
1 UPASI-Tea Research Foundation, Nirar Dam B.P.O, Valparai, India
2 Department of Biotechnology, Karpagam University, Coimbatore, India
3 School of Bio Sciences and Technology, VIT University, Vellore, India

Nucleoside diphosphate kinase (NDPK) operates in the homeostasis of cellular nucleoside triphosphate (NTP) pools and the cytosolic NDPK1 is the main NDPK isoform in plants, accounting for more than 70 % of total NDPK activity in plant. A full length cDNA (697 bp), designated as CsNDPK1 was cloned from Camellia sinensis (L.) O. Kuntze leaves. Sequence analysis of CsNDPK1 shows several motifs, binding and catalytic sites which are highly conserved among other NDPKs. Southern blot analysis revealed that tea genome has two copies of CsNDPK1. Transcription pattern analysis indicated that CsNDPK1 is expressed in all tissues examined, but expressed more in buds than in other organs.

Keywords: Camellia sinensis; cytosolic expression; nucleoside triphosphate pool; sequence analysis; Southern blot
Subjects: cytosolic expression; nucleoside triphosphate pool; sequence analysis; Southern blot; nucleotide diphosphate kinase; expressed sequence tags; PCR; RACE; amino acid sequence; phylogenetic tree; gene expression

Received: March 23, 2010; Accepted: October 19, 2010; Published: March 1, 2012  Show citation

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Prabu, G.R., Thirugnanasambantham, K., Mandal, A.K.A., & Saravanan, A. (2012). Molecular cloning and characterization of nucleoside diphosphate kinase 1 cDNA in tea. Biologia plantarum, 56(1), 140-144. doi: 10.1007/s10535-012-0030-5
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