biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:276-282, 2012 | DOI: 10.1007/s10535-012-0087-1

Frequencies and variation in cytosine methylation patterns in diploid and tetraploid cytotypes of Paspalum notatum

M. P. Rodriguez1,3, G. D. L. Cervigni2, C. L. Quarin3, J. P. A. Ortiz1,3,*
1 Laboratorio de Biología Molecular, Facultad de Ciencias Agrarias, Universidad Nacional de Rosario, Santa Fe, Argentina
2 Centro de Estudios Fotosintéticos y Bioquímicos, CONICET, Santa Fe, Argentina
3 Instituto de Botánica del Nordeste, Facultad de Ciencias Agrarias, Universidad Nacional del Nordeste - CONICET, CC 209, Corrientes, Argentina

Paspalum notatum Flügge is a grass species organized as an agamic complex. The objective of the current research was to survey the frequencies and variation of cytosine methylation at CCGG sequences in diploid and tetraploid genotypes, and to determine the occurrence of methylation changes associated with tetraploidization by using methylation-sensitive amplification polymorphism (MSAP) markers. No differences were found in the average proportions of methylated CCGG sites between cytotypes, but methylation patterns were significantly more variable in tetraploids. In both groups of plants, epigenetic and non-epigenetic variation correlated significantly when compared by Mantel tests. The evaluation of 159 common MSAP markers showed that 18.86 % of them differed in their methylation status in the different ploidies. Dendrogram analysis, reflecting epigenetic distances, showed that the four diploids and one experimentally-obtained sexually-reproducing tetraploid, grouped together. MSAP analysis performed on a diploid plant and its autotetraploid derivative showed that new epialleles emerged after tetraploidization. Sequencing of several MASP markers showed homologies with low copy genes, non-coding sequences and transposon/retrotransposon elements.

Keywords: agamic complex; apomixis; cytosine methylation; MSAP markers
Subjects: agamic complex; apomixis; cytosine methylation; MSAP markers; DNA methylation; phylogenetic tree

Received: May 4, 2010; Accepted: October 19, 2010; Published: June 1, 2012  Show citation

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Rodriguez, M.P., Cervigni, G.D.L., Quarin, C.L., & Ortiz, J.P.A. (2012). Frequencies and variation in cytosine methylation patterns in diploid and tetraploid cytotypes of Paspalum notatum. Biologia plantarum, 56(2), 276-282. doi: 10.1007/s10535-012-0087-1
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