biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:357-361, 2012 | DOI: 10.1007/s10535-012-0098-y

Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis

A. Mitrović1,*, D. Janošević3, S. Budimir2, J. Bogdanović Pristov1
1 Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia
2 Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia
3 Institute of Botany and Botanical Garden "Jevremovac", University of Belgrade, Belgrade, Serbia

Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis from leaf explants were examined. During the early stages of shoot organogenesis there was a decrease in superoxide dismutase (SOD) and an increase in catalase (CAT) activity, and later during organogenesis there was an increase in peroxidase (POD) and polyphenol oxidase (PPO) activity. Two highly regulated turning points may be distinguished regarding activities and isoforms of antioxidative enzymes: the initiation of shoot organogenesis and the shoot bud formation. Our data suggest the role of specific CAT, POD, SOD and PPO isoforms in separate processes during T. bellus direct shoot organogenesis.

Keywords: catalase; peroxidase; polyphenol oxidase; superoxide dismutase
Subjects: catalase; peroxidase; polyphenol oxidase; superoxide dismutase; proteins; benzyladenine; naphthaleneacetic acid; in vitro culture

Received: April 27, 2010; Accepted: October 22, 2010; Published: June 1, 2012  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Mitrović, A., Janošević, D., Budimir, S., & Bogdanović Pristov, J. (2012). Changes in antioxidative enzymes activities during Tacitus bellus direct shoot organogenesis. Biologia plantarum, 56(2), 357-361. doi: 10.1007/s10535-012-0098-y
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Andersone, U., Ievinsh, G.: Changes of morphogenic competence in mature Pinus sylvestris L. buds in vitro. - Ann. Bot. 90: 293-98, 2002. Go to original source...
    2. Asada, K., Yoshikawa, K., Takahashi, M., Maeda, Y., Enmanji, K.: Superoxide dismutase from a blue-green algae Plectonema borianum. - J. biol. Chem. 250: 2801-2807, 1975. Go to original source...
    3. Beauchamp, C., Fridowich, I.: Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. - Anal. Biochem. 44: 276-287, 1971. Go to original source...
    4. Chance, B., Maehly, A.C.: Assay of catalases and peroxidases. - In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Pp. 765-775. Academic Press, New York 1955. Go to original source...
    5. Claiborne, A.: Catalase activity. - In: Greenwald R.A. (ed.): Handbook of Methods for Oxygen Radical Research. Pp. 283-284. CRC Press, Boca Raton 1984.
    6. Dat, J., Vandenabeele, S., Vranová, E., Van Montagu, M., Inze, D., Van Breusegem, F.: Dual action of the active oxygen species during plant stress responses. - Cell. Mol. Life Sci. 57: 779-795, 2000. Go to original source...
    7. Gaspar, T.: Reducing properties and markers of lipid peroxidation in normal and hyperhydrating shoots of Prunus avium L. - J. Plant Physiol. 153: 339-346, 1995. Go to original source...
    8. Gaspar, T., Penel, C., Hagege, D., Grepin, H.: Peroxidases in plant growth, differentiation and developmental processes. - In: Lobarewski, J., Grepin, H., Penel, C., Gaspar, T. (ed.): Biochemical, Molecular and Physiological Aspects of Plant Peroxidases. Pp. 249-280. University M. Curie-Sklodowska, Lublin and University of Geneva, Geneva 1991.
    9. Gillespie, J., Bidochka, M.J., Khachkatourians, G.G.: Separation and characterization of grasshopper hemolymph phenoloxidases by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. - Comp. Biochem. Physiol. 98C: 351-358, 1991. Go to original source...
    10. Gupta, S.D., Datta, S.: Antioxidant enzyme activities during in vitro morphogenesis of gladiolus and the effect of application of antioxidants on plant regeneration. - Biol. Plant. 47: 179-183, 2003/4. Go to original source...
    11. Hendry, G.A.F., Crawford, R.M.M.: Oxygen and environmental stress in plants - an overview. - Proc. roy. Soc. Edinbourgh 102B: 1-10, 1994. Go to original source...
    12. Jackson, P., Ricardo, C.P.P.: The changing peroxidase polymorphism in Lupinus albus during vegetative development. - Aust. J. Plant. Physiol. 25: 261-269, 1998. Go to original source...
    13. Kay, L.E., Basile, D.V.: Specific peroxidase izoenzymes are correlated with organogenesis. - Plant Physiol. 84: 99-105, 1987. Go to original source...
    14. Laemmli, U.K.: Cleavage of structural proteins during assembly of the head of bacteriophage T4. - Nature 227: 680-685, 1970. Go to original source...
    15. Lowry, O., Rosebrough, W., Farr, A., Randall, R.: Protein measurement with the Folin phenol reagent. - J. biol. Chem. 193: 265-275, 1951. Go to original source...
    16. Malda, G., Backhaus, R.A., Martin C.: Alterations in growth and crassulacean acid metabolism (CAM) activity of in vitro cultured cactus. - Plant Cell Tissue Organ Cult. 58: 1-9, 1999. Go to original source...
    17. Mayer, A.M.: Polyphenol oxidases in plants and fungi: going places? - Phytochemistry 67: 2318-2331, 2006. Go to original source...
    18. McCord, J.M., Fridovish, I.: Superoxide dismutase: an enzymatic function for erythrocuprein (hemocuprein). - J. biol. Chem. 244: 6049-6055, 1969. Go to original source...
    19. Meratan, A.A., Ghaffari, S.-M., Niknam, V.: In vitro organogenesis and antioxidant enzymes activity in Acanthophyllum sordidum. - Biol. Plant. 53: 5-10, 2009. Go to original source...
    20. Mitrović, A., Bogdanović, J.: Activities of antioxidative enzymes during Chenopodium rubrum L. ontogenesis in vitro. - Arch. biol. Sci. 60: 223-231, 2008. Go to original source...
    21. Mitrović, A., Vinterhalter, B., Ćulafić, L.: In vitro propagation of Tacitus bellus. - J. Sci. agr. Res. 66: 33-39, 2005.
    22. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures. - Physiol. Plant. 15: 473-497, 1962. Go to original source...
    23. Rajeswari, V., Paliwal, K.: Peroxidase and catalase changes during in vitro adventitious shoot organogenesis from hypocotyls of Albizia odoratissima L.f. (Benth). - Acta Physiol. Plant. 30: 825-832, 2008. Go to original source...
    24. Sujatha, M., Sivaraj, N., Satya Prasad, M.: Biochemical and histological changes during in vitro organogenesis in Jatropha integerrima. - Biol. Plant. 43: 167-171, 2000. Go to original source...
    25. Tang, W., Newton, R.J.: Peroxidase and catalase activities are involved in direct adventitious shoot formation induced by thidiazuron in eastern white pine (Pinus strobus L.) zygotic embryos. - Plant Physiol. Biochem. 43: 760-769, 2005. Go to original source...
    26. Tian, M., Gu, Q., Zhu, M.: The involvement of hydrogen peroxide and antioxidant enzymes in the process of shoot organogenesis of strawberry callus. - Plant Sci. 165: 701-707, 2003. Go to original source...
    27. Vatankhah, E., Niknam, V., Ebrahimzadeh, H.: Activity of antioxidant enzyme during in vitro organogenesis in Crocus sativus. - Biol. Plant. 54: 509-514, 2010. Go to original source...
    28. Woodbury, W., Spencer, A.K., Stahman, M.A.: An improved procedure using ferriccyanide for detecting catalase isozymes. - Anal. Biochem. 44: 301-305, 1971. Go to original source...
    29. Yonghua, Q., Shanglong, Z., Asghar, S., Lingxiao, Z., Qiaoping, Kunsong, C., Changjie, X.: Regeneration mechanism of Toyonoka strawberry under different colour plastic films. - Plant Sci. 168: 1425-1431, 2005. Go to original source...

    Return to the content