biologia plantarum

Biologia plantarum 56:362-364, 2012 | DOI: 10.1007/s10535-012-0099-x

Micropropagation of Pinus peuce

D. Stojičić¹, D. Janošević², B. Uzelac^3,*, V. Čokeša⁴, S. Budimir³

¹ Faculty of Science and Mathematics, University of Niš, Niš, Serbia

² Institute of Botany and Botanical Garden, "Jevremovac" Faculty of Biology, University of Belgrade, Belgrade, Serbia

³ Institute for Biological Research "Siniša Stanković", University of Belgrade, Belgrade, Serbia

⁴ Institute of Forestry, Belgrade, Serbia

In Pinus peuce zygotic embryo culture grown on Gresshoff and Doy (1972; GD) basal medium, 2.22 μM benzyladenine (BA) was superior in promoting adventitious bud induction during 4 weeks comparing to kinetin or BA + kinetin. Shoot elongation was achieved on half-strength GD medium devoid of plant growth regulators and containing activated charcoal. Pulse treatment with 1 mM indole-3-butyric acid (IBA) for 2 h, followed by transfer to half-strength GD medium, produced the most efficient rooting. Rooted shoots were transplanted to the greenhouse and plantlets continued to grow and developed into phenotypically normal plants. Up to 10 plants per explant can be obtained within 36 weeks from culture initiation.

Keywords: adventitious buds; benzyladenine; conifers; Macedonian pine; micropropagation

Subjects: adventitious buds; benzyladenine; abiotic stress; in vitro culture; benzyadenine; kinetin

Received: July 21, 2010; Accepted: October 26, 2010; Published: June 1, 2012  Show citation