biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:373-376, 2012 | DOI: 10.1007/s10535-012-0102-6

Monitoring of cultivar identity in micropropagated olive plants using RAPD and ISR markers

A. R. Leva1,*, R. Petruccelli1
1 CNR-IVALSA, Sesto Fiorentino, Firenze, Italy

Randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers were applied to assess the genetic stability of micropropagated olive (Olea europaea L. cv. Maurino) plants regenerated by axillary buds. Initial olive explants, isolated from one donor tree, were multiplied on Murashige and Skoog medium for 12 repeated subcultures. A total of 40 RAPD and 10 ISSR markers resulted in 301 distinct and reproducible band classes showing homogeneous RAPD and ISSR patterns. The amplification products revealed genetic stability among the micropropagated plants and between them and the donor plant. The results demonstrate the genetic stability of nine year old mature micropropagated olive plants cultured in field, and corroborated the fact that axillary multiplication is the safest mode for multiplication of true to type plants.

Keywords: genetic stability; Olea europaea
Subjects: genetic stability; RAPD; ISSR; in vitro culture

Received: January 31, 2011; Accepted: May 23, 2011; Published: June 1, 2012  Show citation

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Leva, A.R., & Petruccelli, R. (2012). Monitoring of cultivar identity in micropropagated olive plants using RAPD and ISR markers. Biologia plantarum, 56(2), 373-376. doi: 10.1007/s10535-012-0102-6
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