biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:401-408, 2012 | DOI: 10.1007/s10535-012-0063-9

Evaluation of different embryogenic systems for production of true somatic embryos in Arabidopsis

K. Nowak1, B. Wojcikowska1, K. Szyrajew1, M. D. Gaj1,*
1 Department of Genetics, University of Silesia, Katowice, Poland

Somatic embryogenesis (SE) in Arabidopsis was induced using various systems, including auxin treatment of in vitro cultured explants (immature zygotic embryos, IZEs) and transgenic plants overexpressing embryogenesis-related transcription factors, e.g. LEC2 together with the GUS reporter gene under control of the auxin-induced DR5 promoter. The study indicated that the SE-systems used gave different embryogenic capacities for the production of true embryos. The highest ratio of true embryos (75 %) was found among embryo-like structures in transgenic seedlings overexpressing LEC2. Analysis of in vitro induced SE systems indicated that in somatic embryos produced in response to exogenous auxin treatment the formation of root poles is frequently disturbed. A lack of a properly formed root meristem was observed in 35-80 % of in vitro induced somatic embryos, in dependence on auxin concentration and duration of treatment.

Keywords: DR5::GUS; LEC2 overexpression; reporter gene analysis; root formation in vitro
Subjects: DR5::GUS; LEC2 overexpression; reporter gene analysis; transgenic plants; somatic embryogenesis; immature zygotic embryos; callus; GUS expression; in vitro rooting; in vitro culture; indoleacetic acid

Received: April 10, 2011; Accepted: July 13, 2011; Published: September 1, 2012  Show citation

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Nowak, K., Wojcikowska, B., Szyrajew, K., & Gaj, M.D. (2012). Evaluation of different embryogenic systems for production of true somatic embryos in Arabidopsis. Biologia plantarum, 56(3), 401-408. doi: 10.1007/s10535-012-0063-9
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    References

    1. Barry-Etienne, D., Bertrand, B., Vasquez, N., Etienne, H.: Comparison of somatic embryogenesis-derived coffee (Coffea arabica L.) plantlets regenerated in vitro or ex vitro: morphological, mineral and water characteristics. - Ann. Bot. 90: 77-85, 2002. Go to original source...
    2. Bassuner, B.M., Lam, R., Lukowitz, W., Yeung, E.C.: Auxin and root initiation in somatic embryos of Arabidopsis. - Plant Cell Rep. 26: 1-11, 2007. Go to original source...
    3. Benfey, P.N., Bennett, M., Schiefelbein, J.: Getting to the root of plant biology: impact of the Arabidopsis genome sequence on root research. - Plant J. 61: 992-1000, 2010. Go to original source...
    4. Boutilier, K., Offringa, R., Sharma, V.K., Kieft, H., Ouellet, T., Zhang, L., Hattori, J., Liu, C.M., Van Lammeren, A.A.M., Miki, B.L.A., Custers, J.B.M., Van Lookeren Compagne, M.M.: Ectopic expression of BABY BOOM triggers a conversion from vegetative to embryonic growth. - Plant Cell 14: 1737-1749, 2002. Go to original source...
    5. Carredoira, E., Ballester, A., Vieitez, A.M.: Proliferation, maturation and germination of Castanea sativa Mill. somatic embryos originated from leaf explants. - Ann. Bot. 92: 129-136, 2003. Go to original source...
    6. Casson, S., Spencer, M.M., Walker, W., Lindsey, K.: Laser capture microdissection for the analysis of gene expression during embryogenesis of Arabidopsis. - Plant J. 42: 111-123, 2005. Go to original source...
    7. Debnath, M., Prasad, G.B.K.S., Bisen, P.S.: Reporter gene. In: Debnath, M., Prasad, G.B.K.S., Bisen, P.S (ed.): Molecular Diagnostics: Promises and Possibilities. Pp. 71-84. Springer, Dordrecht - Heidelberg - London - New York 2010. Go to original source...
    8. Faure, O., Aarrouf, J., Nougarede, A.: Ontogenesis, differentiation and precocious germination in anther-derived somatic embryos of grapevine (Vitis vinifera L.): embryonic organogenesis. - Ann. Bot. 78: 29-37, 1996. Go to original source...
    9. Feher, A., Pasternak, T.P., Dudits, D.: Transition of somatic plant cells to an embryogenic state. - Plant Cell Tissue Organ Cult. 74: 201-228, 2003. Go to original source...
    10. Feldman, K.A., Marks, M.D.: Rapid and efficient regeneration of plants from explants of Arabidopsis thaliana. - Plant Sci. 47:63-69, 1986. Go to original source...
    11. Friml, J., Vieten, A., Sauer, M., Weijers, D., Schwarz, H., Hamann, T., Offringa, R., Jürgens, G.: Efflux-dependent auxin gradients establish the apical-basal axis of Arabidopsis. - Nature 426: 147-153, 2003. Go to original source...
    12. Gaj, M.D.: Direct somatic embryogenesis as a rapid and efficient system for in vitro regeneration of Arabidopsis thaliana (L.) Heynh. - Plant Cell Tissue Organ Cult. 64: 39-46, 2001. Go to original source...
    13. Gaj, M.D.: Somatic embryogenesis and plant regeneration in the culture of Arabidopsis thaliana (L.) Heynh. immature zygotic embryos. - In: Thorpe, T.A., Yeung E.C (ed.): Plant Embryo Culture. Pp. 257-265. Humana Press, Totowa 2011. Go to original source...
    14. Gamborg, O.L., Miller, L.A., Ojima, K.: Nutrient requirement of suspension cultures of soybean root cells. - Exp. cell. Res. 50: 151-158, 1968. Go to original source...
    15. George, E.F., Debergh, P.C.: Micropropagation: uses and methods. - In: George, E.F., Hall, M.A., De Klerk, G.J. (ed.): Plant Propagation by Tissue Culture. 3rd Ed. Pp. 29-65. Springer, Dordrecht 2008. Go to original source...
    16. Griga, M.: Morphology and anatomy of Pisum sativum somatic embryos. - Biol. Plant. 45: 173-182, 2002. Go to original source...
    17. Hadfi, K., Speth, V., Neuhaus, G.: Auxin-induced developmental patterns in Brassica juncea embryos. - Development 125: 879-887, 1998. Go to original source...
    18. Harding, E.W., Tang, W., Nichols, K.W., Fernandez, D.E., Perry, S.E.: Expression and maintenance of embryogenic potential is enhanced through constitutive expression of AGAMOUS-Like 15. - Plant Physiol. 133: 653-663, 2003. Go to original source...
    19. Ikeda-Iwai, M., Umehara, M., Satoh, S., Kamada, H.: Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryos. - J. exp. Bot. 53: 1575-1580, 2002. Go to original source...
    20. Jiang, K., Feldman, L.J.: Root meristem establishment and maintenance: the role of auxin. - J. Plant Growth Regul. 21: 432-440, 2005a. Go to original source...
    21. Jiang, K., Feldman, L.J.: Regulation of root apical meristem development. - Annu. Rev. cell. dev. Biol. 21: 485-509, 2005b. Go to original source...
    22. Koprivova, A., Mugford, S.T., Kopriva, S.: Arabidopsis root growth dependence on glutathione is linked to auxin transport. - Plant Cell Rep. 29: 1157-1167, 2010. Go to original source...
    23. Kurczyñska, E.U., Gaj, M.D., Ujczak, A., Mazur, E.: Histological analysis of direct somatic embryogenesis in Arabidopsis thaliana (L.) Heynh. - Planta 226: 619-628, 2007. Go to original source...
    24. Kyozuka, J.: Control of shoot and root meristem function by cytokinin. - Curr. Opin. Plant Biol. 10: 442-446, 2007. Go to original source...
    25. Ledwon, A., Gaj, M.D.: LEAFY COTYLEDON2 gene expression and auxin treatment in relation to embryogenic capacity of Arabidopsis somatic cells. - Plant Cell Rep. 28: 1677-1688, 2009. Go to original source...
    26. Lotan, T., Ohtom, M., Matsudaira Y.K., West, M.A.L., Lo, R., Kwong, R.W., Yamagishi, K., Fischer, R.L., Goldberg, R.B., Harada, J.J.: Arabidopsis LEAFY COTYLEDON1 is sufficient to induce embryo development in vegetative cells. - Cell 93: 1195-1205, 1998. Go to original source...
    27. Madden, J.I., Jones, C.S., Auer, C.A.: Modes of regeneration in Pelargonium × hortorum (Geraniaceae) and three closely related species. - In Vitro cell. dev. Biol. Plant 41: 37-46, 2005. Go to original source...
    28. Mendes A.F.S., Cidade L.C., Otoni W.C., Soares-Filho W.S., Costa M.G.C.: Role of auxins, polyamines and ethylene in root formation and growth in sweet orange. - Biol. Plant 55: 375-378. 2011. Go to original source...
    29. Mordhorst, A.P., Voerman, K.J., Hartog, M.V., Meijer, E.A., van Went, J., Koornneef, M., de Vries, S.C.: Somatic embryogenesis in Arabidopsis thaliana is facilitated by mutations in genes repressing meristematic cell divisions. - Genetics 149: 549-563, 1998. Go to original source...
    30. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco cultures. - Plant Physiol. 15: 473-492, 1962. Go to original source...
    31. Nolan, K.E., Kurdyukov, S., Rose, R.J.: Expression of the SOMATIC EMBRYOGENESIS RECEPTORLIKE KINASE1 (SERK1) gene is associated with developmental change in the life cycle of the model legume Medicago truncatula. - J. exp. Bot. 60: 1759-1771, 2009. Go to original source...
    32. Pillon, E., Terzi, M., Baldan, B., Mariani, P., Schiavo, F.L.: A protocol for obtaining embryogenic cell lines from Arabidopsis. - Plant J. 9: 573-577, 1996. Go to original source...
    33. Raghavan, V.: Role of 2,4-dichlorophenoxyacetic acid (2,4-D) in somatic embryogenesis on cultured zygotic embryos of Arabidopsis: cell expansion, cell cycling, and morphogenesis during continuous exposure of embryos to 2,4-D. - Amer. J. Bot. 91: 1743-1756, 2004. Go to original source...
    34. Raghavan, V.: Somatic embryogenesis. - In: Murch, S.J., Saxena, P.K. (ed.): Journey of a Single Cell to a Plant. Pp. 203-226. Science Publishers, Enfield 2005.
    35. Sabatini, S., Beis, D., Wolkenfelt, H., Murfett, J., Giulfoyle, T., Malamy, J., Benfey, P., Leyder, O., Bechtold, N., Weisbeek, P., Scheres, B.: An auxin-dependent distal organizer of pattern and polarity in the Arabidopsis root. - Cell 99: 463-472, 1999. Go to original source...
    36. Salaj, J., Petrovská, B., Obert, B., Pretová, A.: Histological study of embryo-like structures initiated from hypocotyl segments of flax (Linum usitatissimum L.). - Plant Cell Rep. 24: 590-595, 2005. Go to original source...
    37. Sharma, S.K., Millam, S.: Somatic embryogenesis in Solanum tuberosum L.: a histological examination of key developmental stages. - Plant Cell Rep. 23: 115-119, 2004. Go to original source...
    38. Smith, Z.R., Long, J.A.: Control of Arabidopsis apical-basal embryo polarity by antagonistic transcription factors. - Nature 464: 423-427, 2010. Go to original source...
    39. Steward, F.C., Mapes, M.O., Mears, K.: Growth and organized development of cultured cells. II. Organization in cultures grown from freely suspended cells. - Amer. J. Bot. 45: 705-708, 1958. Go to original source...
    40. Stone, S.L., Kwong, L.W., Ye, K.M., Pelletier, J., Lepiniec, L., Fischer, R.L., Goldberg, R.B., Harada, J.J.: LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development. - Proc. nat. Acad. Sci. USA 98: 11806-11811, 2001. Go to original source...
    41. Stone, S.L., Braybrook, S.A., Paula, S.L., Kwong, L.W., Meuser, J., Pelletier, J., Hsieh, T.F., Fischer, R.L., Goldberg, R.B., Harada, J.J.: Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity: Implications for somatic embryogenesis. - Proc. nat. Acad. Sci. USA 105: 3151-3156, 2008. Go to original source...
    42. Su, Y.H., Zhao, X.Y., Liu, Y.B., Zhang, C.L., O'Neil, S.D., Zhang, X.S.: Auxin-induced WUS expression is essential for embryonic stem cell renewal during somatic embryogenesis in Arabidopsis. - Plant J. 59: 448-460, 2009. Go to original source...
    43. Tzafrir, I., Pena-Muralla, R., Dickerman, A., Berg, M., Rogers, R., Hutchens, S., Sweeney, T.C., McElver, J., Aux, G., Patton, D., Meinke, D.: Identification of genes required for embryo development in Arabidopsis. - Plant Physiol. 135: 1206-1220, 2004. Go to original source...
    44. Ulmasov, T., Murfett, J., Hagen, G., Guilfoyle, T.J.: Aux/IAA proteins repress expression of reporter genes containing natural and highly active synthetic auxin response elements. - Plant Cell 9: 1963-1971, 1997. Go to original source...
    45. Vasil, V., Lu, C.Y., Vasil, I.K.: Histology of somatic embryogenesis in cultured immature embryos of maize (Zea mays L.). - Protoplasma 127: 1-8, 1985. Go to original source...
    46. Wang, X., Niu, Q.W., Teng, C., Li, C., Mu, J., Chua, N.H., Zuo, J.: Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis. - Cell Res.: 1-12, 2008. Go to original source...
    47. Werner, T., Motyka, V., Laucou, V., Smets, R., Van Onckelen, H., Schmülling, T.: Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity. - Plant Cell 15: 2532-2550, 2003. Go to original source...
    48. Zimmerman, L.: Somatic embryogenesis: a model for early development in higher plants. - Plant Cell 5: 1411-1423, 1993. Go to original source...
    49. Zuo, J., Niu, Q.W., Frugis, G., Chua, N.H.: The WUSCHEL gene promotes vegetative-to-embryonic transition in Arabidopsis. - Plant J. 30: 349-359, 2002. Go to original source...

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