biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:528-536, 2012 | DOI: 10.1007/s10535-012-0064-8

The role of non-symbiotic haemoglobin and nitric oxide homeostasis in waterlogging tolerance in Vigna species

R. K. Sairam1,*, K. Dharmar1, V. Chinnusamy2, S. Lekshmy1, R. Joshi1, P. Bhattacharya1
1 Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, India
2 Department of Botany and Plant Sciences, University of California, Riverside, USA

The objective of this study was to examine the role of nitrate reductase, nitric oxide and non-symbiotic hemoglobin in imparting waterlogging tolerance in mung bean genotypes. Experiment was conducted with two cultivated mung bean [Vigna radiata (L.) Wilczek] genotypes T 44 (tolerant) and Pusa Baisakhi (susceptible) and a highly tolerant wild species Vigna luteola (Jacq.) Benth. The content of nitric oxide increased up to 6 d of waterlogging in Vigna luteola and T 44, and up to 4 d of treatment in Pusa Baisakhi. Increase in nitrate reductase (NR) activity was observed only up to 4 d of waterlogging in Vigna luteola and T 44, and up to 2 d of treatment in Pusa Baisakhi, and thereafter the activity decreased in all the genotypes. The increase in NO content and NR activity was greater in Vigna luteola and T 44 than in Pusa Baisakhi. Non-symbiotic hemoglobin (NSHb) and cNR mRNA expressions were observed only in waterlogging treated roots of Vigna luteola and T 44, while very little expression was observed in control plants of Vigna luteola and T 44, and in control and waterlogged plants of Pusa Baisakhi. PCR bands of Hb and cNR were cloned, and nucleotide and deduced amino acid sequences were obtained and conserved regions and domains were identified using database.

Keywords: gene expression; mung bean; nitrate reductase; Vigna luteola; Vigna radiata
Subjects: gene expression; nitrate reductase; non-symbiotic haemoglobin; nitric oxide; waterlogging; PCR; nucleotide sequence; amino acid sequence

Received: October 29, 2010; Accepted: August 30, 2011; Published: September 1, 2012  Show citation

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Sairam, R.K., Dharmar, K., Chinnusamy, V., Lekshmy, S., Joshi, R., & Bhattacharya, P. (2012). The role of non-symbiotic haemoglobin and nitric oxide homeostasis in waterlogging tolerance in Vigna species. Biologia plantarum, 56(3), 528-536. doi: 10.1007/s10535-012-0064-8
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