biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:537-544, 2012 | DOI: 10.1007/s10535-012-0107-1

Oligogalacturonides stimulate antioxidant system in alfalfa roots

D. Camejo1, M. C. Martí1, E. Olmos1, W. Torres2, F. Sevilla1, A. Jiménez1,*
1 Department of Stress Biology and Plant Pathology, CEBAS-CSIC, Murcia, Spain
2 Department of Plant Physiology and Biochemistry, INCA, S. José de las Lajas, La Habana, Cuba

Alfalfa (Medicago sativa L.) roots were treated with 50 and 100 μg cm-3 of oligogalacturonide (OGA) solutions with a degree of polymerization between 7 and 15. Changes in the activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POX), ascorbate peroxidase (APX), monodehydroascorbate reductase (MDHAR) and dehydroascorbate reductase (DHAR) as well as ascorbate (ASC) content were determined in crude extract of alfalfa roots after 30, 60 and 120 min of treatment. An increase in the SOD activity was observed in roots treated with 50 and 100 μg cm-3 OGA, which could be related to its O2 .- scavenging function. As concern H2O2 scavenging, CAT activity was increased in the first 30 min by both OGA concentrations, while POX was a key enzyme at higher OGA concentration and treatment duration. ASC content firstly increased upon exposure to high OGA concentration, and then decreased after longer treatment while low OGA concentration had no effect on ASC content.

Keywords: ascorbate; ascorbate peroxidase; catalase; dehydroascorbate reductase; monodehydroascorbate reductase; reactive oxygen species; peroxidase; superoxide dismutase
Subjects: ascorbate; ascorbate peroxidase; catalase; dehydroascorbate reductase; monodehydroascorbate reductase; reactive oxygen species; peroxidase; superoxide dismutase; oligogalacturonide

Received: October 25, 2010; Accepted: March 16, 2011; Published: September 1, 2012  Show citation

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Camejo, D., Martí, M.C., Olmos, E., Torres, W., Sevilla, F., & Jiménez, A. (2012). Oligogalacturonides stimulate antioxidant system in alfalfa roots. Biologia plantarum, 56(3), 537-544. doi: 10.1007/s10535-012-0107-1
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