biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:657-666, 2012 | DOI: 10.1007/s10535-012-0120-4

Early gene expression in the walnut tree occurring during stimulation of leaf hydraulic conductance by irradiance

K. Ben Bâaziz1,2,3, D. Lopez1,2, S. Bouzid3, H. Cochard1,2, J. -S. Venisse1,2, S. Sakr1,2,4,*
1 Clermont-Université, UMR 547 PIAF, Aubière Cedex, France
2 UMR 547 PIAF, INRA, Clermont-Ferrand, France
3 Laboratoire de Biologie végétale, Faculté des Sciences de Tunis, Campus Universitaire, Tunis, Tunisia
4 Agrocampus Ouest, Centre d'Angers, UMR SAGAH, Angers Cedex, France

Leaf hydraulic conductance (Kleaf) plays a significant part in plant-water regulation. In walnut leaves, Kleaf is stimulated by irradiance and closely relates to the accumulation of JrPIP2s aquaporin transcripts, but it is independent of stomatal aperture. To provide an insight into the early molecular events occurred during light-induced Kleaf, a large-scale transcriptomic analysis consisting of the cDNA-amplified fragment length polymorphism (AFLP) was carried out on walnut leaves maintained under irradiance or in darkness. Of the total 12 000 transcript-derived fragments (TDFs) obtained using cDNA-AFLP with 128 primer pairs, 187 TDFs were selected after sequencing, and only 93 (49 %) that had been ascribed known functions through BLAST searching of the GenBank databases. Most of these TDFs correspond to genes whose protein products are involved in cellular regulation (57.9 %) and global metabolism (39.8 %). To validate cDNA-AFLP expression patterns, 30 TDFs were further analyzed using real-time quantitative polymerase chain reaction. Moreover, exposure of leaves to irradiance was accompanied by the modification of the Ca2+-signaling pathway, ubiquitin-proteasome pathway, vesicle trafficking process and expression of multiple transcription factors.

Keywords: aquaporin; cDNA-AFLP; Juglans regia; leaf water transport; plasma membrane intrinsic protein; qRT-PCR
Subjects: gene expression; leaf hydraulic conductance; water transport; irradiance; plasma membrane; aquaporins; cDNA-AFLP; transcript-derived fragments

Received: May 17, 2010; Accepted: April 30, 2011; Published: December 1, 2012  Show citation

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Ben Bâaziz, K., Lopez, D., Bouzid, S., Cochard, H., Venisse, J.-S., & Sakr, S. (2012). Early gene expression in the walnut tree occurring during stimulation of leaf hydraulic conductance by irradiance. Biologia plantarum, 56(4), 657-666. doi: 10.1007/s10535-012-0120-4
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