biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:675-682, 2012 | DOI: 10.1007/s10535-012-0245-5

Ascorbate deficient semi-dwarf asfL1 mutant of Lathyrus sativus exhibits alterations in antioxidant defense

D. Talukdar1,*
1 Department of Botany, R.P.M. College, University of Calcutta, Uttarpara, India

An ascorbate-deficient semi-dwarf mutant asfL-1 was detected in 250 Gy γ-ray treated grass pea (Lathyrus sativus L.) cv. BioR-231. The mutant contained only 42 % of leaf and 20 % of root ascorbate content of mother control (MC). I investigated the possible causes of ascorbate deficiency and its effect on growth and antioxidant defense in control and 150 mM NaCl-treated seedling after 60 d growth period. Ascorbate deficiency was due to significant reduction in activities of monodehydroascorbate reductase and dehydroascorbate reductase as well as increase in ascorbate oxidase, leading to considerable decrease in redox state. Despite low ascorbate pool and decrease in ascorbate peroxidase activity, shoot and root biomass production in asfL-1 mutant were similar to MC plants, even at NaCl treatment. High accumulation of glutathione (GSH) coupled with high activities of GSH reductase, catalase, GSH peroxidase and peroxidase in both tissues of the mutant permitted efficient recycling of GSH and scavenging of H2O2 through well integrated catalase/peroxidase system, despite high superoxide dismutase activity under NaCl treatment. The collapse of this system led to inhibition of growth in NaCl-treated mother plants. Together, the results suggested that asfL-1 plants undertook a major reshuffle in its antioxidant defense machinery, which effectively counterbalanced the negative impact of ascorbate deficiency and remained unperturbed by NaCl treatment to maintain normal growth and biomass production.

Keywords: ascorbate-glutathione cycle; catalase; dehydroascorbate reductase; glutathione reductase; glutathione peroxidase; grass pea; hydrogen peroxide; oxidative stress
Subjects: ascorbate; mutant; glutathione; NaCl; superoxide dismutase; monodehydroascorbate reductase; dehydroascorbate reductase; glutathione reductase; ascorbate oxidase; ascorbate peroxidase; catalase; peroxidases; hydrogen peroxide; malondialdehyde; glutathione peroxidase

Received: March 21, 2011; Accepted: August 3, 2011; Published: December 1, 2012  Show citation

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Talukdar, D. (2012). Ascorbate deficient semi-dwarf asfL1 mutant of Lathyrus sativus exhibits alterations in antioxidant defense. Biologia plantarum, 56(4), 675-682. doi: 10.1007/s10535-012-0245-5
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    References

    1. Aebi, H.: Catalase in vitro. - Methods Enzymol. 105: 121-126, 1984. Go to original source...
    2. Agarwal, S., Pandey, V.: Antioxidant enzyme responses to NaCl stress in Cassia angustifolia. - Biol. Plant. 48: 555-560, 2004. Go to original source...
    3. Arrigoni, O.: Ascorbate system in plant development. - J. Bioenerg. Biomembr. 26: 407-419, 1994. Go to original source...
    4. Asada, K.: Ascorbate peroxidase - a hydrogen peroxidescavenging enzyme in plants. - Physiol. Plant. 85: 235-241, 1992. Go to original source...
    5. Beyer, W.F., Fridovich, I.: Assaying for superoxide dismutase activity: some large consequences of minor changes in conditions. - Ann. Biochem. 161: 559-566, 1987. Go to original source...
    6. Carmagnol, F., Sinet, P.M., Jerome, H.: Selenium-dependent and non-selenium-dependent glutathione peroxidases in human tissue extracts. - Biochim. biophys. Acta 759: 49-57, 1983. Go to original source...
    7. Cheeseman, J.M.: Hydrogen peroxide concentrations in leaves under natural conditions. - J. exp. Bot. 10: 2435-2444, 2006. Go to original source...
    8. Conklin, P.L., Williams, E.H., Last, R.L.: Environmental stress sensitivity of an ascorbic acid-deficient Arabidopsis mutant. - Proc. nat. Acad. Sci. USA 3: 9970-9974, 1996. Go to original source...
    9. Dalton, D.A., Langeberg, L., Treneman, N.C.: Correlations between the ascorbate-glutathione pathway and effectiveness in legume root nodules. - Physiol. Plant. 87: 365-370, 1993. Go to original source...
    10. De Pinto, M.C., De Gara, L.: Changes in the ascorbate metabolism of apoplastic and symplastic spaces are associated with cell differentiation. - J. exp. Bot. 55: 2559-2569, 2004. Go to original source...
    11. Díaz-Vivancos, P., Barba-Espín, G., Clemente-Moreno, M.J., Hernández, J.A.: Characterization of antioxidant system during the vegetative development of pea plants. - Biol. Plant. 54: 76-82, 2010. Go to original source...
    12. Drotar, A., Phelps, P., Fall, R.: Evidence for glutathione peroxidase activities in cultured plant cells. - Plant Sci. 42: 35-40, 1985. Go to original source...
    13. Foyer, C.H., Noctor, G.: Ascorbate and glutathione: the heart of the redox hub. - Plant Physiol. 155: 2-18, 2011. Go to original source...
    14. Griffith, O.W.: Glutathione and glutathione disulfide. - In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Pp. 521-529. Verlagsgesellschaft, Weinheim 1985.
    15. Halliwell, B.: Reactive species and antioxidants: redox biology is a fundamental theme of aerobic life. - Plant Physiol. 141: 312-322, 2006. Go to original source...
    16. Hernández, J.A., Jiménez, A., Mullineaux, P., Sevilla, F.: Tolerance of pea (Pisum sativum L.) to long-term salt stress is associated with induction of antioxidant defences. - Plant Cell Environ. 23: 853-862, 2000. Go to original source...
    17. Hernández, M., Fernandez-Garcia, N., Díaz-Vivancos, P., Olmos, E.: A different role for hydrogen peroxide and the antioxidative system under short and long salt stress in Brassica oleracea roots. - J. exp. Bot. 61: 521-535, 2010. Go to original source...
    18. Law, M.Y., Charles, S.A., Halliwell, B.: Glutathione and ascorbic acid in spinach (Spinacia oleracea) chloroplast. The effect of hydrogen peroxide and paraquat. - Biochem. J. 210: 899-903, 1983. Go to original source...
    19. Lin, K.H., Pu, S.F.: Tissue- and genotype-specific ascorbate peroxidase expression in sweet potato in response to salt stress. - Biol. Plant. 54: 664-670, 2010. Go to original source...
    20. Mahdavi, B., Sanavy, S.A.M.M.: Germination and seedling growth in grass pea (Lathyrus sativus L.) cultivars under salinity conditions. - Pak. J. Biol. Sci. 10: 273-279, 2007. Go to original source...
    21. Maia, J.M., Costa de Macedo, C.E., Voigt, E.L., Freitas, J.B.S., Silveira, J.A.G.: Antioxidant enzymatic protection in leaves of two contrasting cowpea cultivars under salinity. - Biol. Plant. 54: 159-163, 2010. Go to original source...
    22. Mallik, S., Nayak, M., Sahu, B.B., Panigrahi, A.K., Shaw, B.P.: Response of antioxidant enzymes to high NaCl concentration in different salt-tolerant plants. - Biol. Plant. 55: 191-195, 2011. Go to original source...
    23. Maughan, S., Foyer, C.H.: Engineering and genetic approaches to modulating the glutathione network in plants. - Physiol. Plant. 126: 382-397, 2006. Go to original source...
    24. Miao, Y., Lv, D., Wang, P., Wang, X-C., Chen, J., Miao, C., Song, C-P.: An Arabidopsis glutathione peroxidase functions as both a redox transducer and a scavenger in abscisic acid and drought stress responses. - Plant Cell 18: 2749-2766, 2006. Go to original source...
    25. Miller, G., Suzuki, N., Rizhsky, L., Hegie, A., Koussevitzky, S., Mittler, R.: Double mutants deficient in cytosolic and thylakoid ascorbate peroxidase reveal a complex mode of interaction between reactive oxygen species, plant development, and response to abiotic stresses. - Plant Physiol. 144: 1777-1785, 2007. Go to original source...
    26. Nakano, Y., Asada, K.: Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplast. - Plant Cell Physiol. 22:867-880, 1981.
    27. Navrot, N., Collin, V., Gualberto, J., Gelhaye, E., Hirasawa, M., Rey, P., Knaff, D.B., Issakidis, E., Jacquot, J.P., Rouhier, N.: Plant glutathione peroxidases are functional peroxiredoxins distributed in several subcellular compartments and regulated during biotic and abiotic stresses. - Plant Physiol. 142: 1364-1379, 2006. Go to original source...
    28. Niknam, V., Razavi, N., Ebrahimzadeh, H., Sharifizadeh, B.: Effect of NaCl on biomass, protein and proline contents, and antioxidant enzymes in seedlings and calli of two Trigonella species. - Biol. Plant. 50: 591-596, 2006. Go to original source...
    29. Noctor, G., Gomez, L., Vanacker, H., Foyer, C.H.: Interactions between biosynthesis, compartmentation and transport in the control of glutathione homeostasis and signaling. - J. exp. Bot. 53: 1283-1504, 2002. Go to original source...
    30. Passardi, F., Penel, C., Dunand, C.: Performing the paradoxical: how plant peroxidases modify the cell wall. - Trends. Plant Sci. 9: 534-540, 2004. Go to original source...
    31. Saher, S., Piqueras, A., Hellin, E., Olmos, E.: Hyperhydricity in micropropagated carnation shoots: the role of oxidative stress. - Physiol. Plant. 120: 152-161, 2004. Go to original source...
    32. Takahama, I., Oniki, T.: Effects of ascorbate on the oxidation of derivatives of hydroxycinnamic acid and the mechanism of oxidation of sinapic acid by cell wall-bound peroxidases. - Plant Cell Physiol. 35: 593-600, 1994. Go to original source...
    33. Talukdar, D.: Development of cytogenetic stocks through induced mutagenesis in grass pea (Lathyrus sativus): Current status and future prospects in crop improvement. - Grain Legume 54: 30-31, 2009.
    34. Talukdar, D.: Flower and pod production, abortion, leaf injury, yield and seed neurotoxin levels in stable dwarf mutant lines of grass pea (Lathyrus sativus L.) differing in salt stress responses. - Int. J. Curr. Res. 2: 46-54, 2011a.
    35. Talukdar, D.: Effect of arsenic-induced toxicity on morphological traits of Trigonella foenum-graecum L. and Lathyrus sativus L during germination and early seedling growth. - Curr. Res. J. biol. Sci. 3: 116-123, 2011b.
    36. Talukdar, D.: Isolation and characterization of NaCl-tolerant mutations in two important legumes, Clitoria ternatea L. and Lathyrus sativus L.: induced mutagenesis and selection by salt stress. - J. med. Plants Res. 5: 3619-3628, 2011c.
    37. Talukdar, D., Biswas, A.K.: An induced internode mutant in grass pea. - In: Manna, G.K., Roy, S.C. (ed.): Perspectives in Cytology and Genetics. Vol. 12. Pp. 267-272. AICCG Publ., Kalyani 2006.
    38. Vaz Patto, M., Skiba, B., Pang, E., Ochatt, S., Lambein, F., Rubiales, F.: Lathyrus improvement for resistance against biotic and abiotic stresses: from classical breeding to marker assisted selection. - Euphytica 147:133-147, 2006. Go to original source...
    39. Veljovic-Jovanovic, S.D., Pignocchi, C., Noctor, G., Foyer, C.H.: Low ascorbic acid in the vtc-1 mutant of Arabidopsis is associated with decreased growth and intracellular redistribution of the antioxidant system. - Plant Physiol. 127: 426-435, 2001. Go to original source...
    40. Vernoux, T., Wilson, R.C., Seeley, K.A., Reichheld, J.P., Muroy, S., Brown, S., Maughan, S.C., Cobbett, C.S., Van Montagu, M., Inzè, D., May, M.J., Sung, Z.R.: The ROOT MERISTEMLESS/CADMIUM SENSITIVE2 gene defines a glutathione-dependent pathway involved in initiation and maintenance of cell division during postembryonic root development. - Plant Cell 12: 97-110, 2000. Go to original source...

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