biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 56:693-698, 2012 | DOI: 10.1007/s10535-012-0143-x

Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars

C. Kayihan1, F. Eyidogan2,*, N. Afsar1, H. A. Oktem1, M. Yucel1
1 Department of Biology, Middle East Technical University, Ankara, Turkey
2 Faculty of Education, Baskent University, Ankara, Turkey

The transcript levels and activities of the superoxide dismutase isoenzyme (Cu/ZnSOD) were assessed in winter (Tarm-92) and spring (Zafer-160) barley cultivars during cold acclimation, freezing stress and after rewarming. Leaf Cu/ZnSOD activity and Cu/ZnSOD expression level were not significantly changed during cold acclimation. The Cu/ZnSOD expression increased evidently at mild freezing stress (-3 °C; F1), while Cu/ZnSOD1 activity did not show any response and Cu/ZnSOD2 activity decreased continuously during F1 and F2 (-7 °C) in Tarm-92. On the other hand, root Cu/ZnSOD2 activity was in accordance with Cu/ZnSOD expression in Zafer-160 after F2 treatment. Rewarming periods did not cause any significant changes in the Cu/ZnSOD activity and expression of Cu/ZnSOD in both cultivars when compared to freezing stresses. These results showed that freezing stress can regulate differently Cu/ZnSOD transcription and enzyme activity.

Keywords: antioxidant enzymes; Hordeum vulgare; low temperature; rewarming
Subjects: superoxide dismutase; temperature - low; cold acclimation; PCR; gene expression

Received: October 19, 2011; Accepted: January 11, 2012; Published: December 1, 2012  Show citation

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Kayihan, C., Eyidogan, F., Afsar, N., Oktem, H.A., & Yucel, M. (2012). Cu/Zn superoxide dismutase activity and respective gene expression during cold acclimation and freezing stress in barley cultivars. Biologia plantarum, 56(4), 693-698. doi: 10.1007/s10535-012-0143-x
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