biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:771-774, 2012 | DOI: 10.1007/s10535-012-0141-z

Stomatal NH3 compensation point and its metabolic regulation in senescence phenotypes of Nicotiana tabacum

W. J. Duan1,2, T. Z. Yang1,*, Y. Dai2, D. L. Li2, X. Q. Zhang1, H. B. Liu1, N. Li2, C. G. Wang2
1 Henan Agricultural University, Zhengzhou, P.R.China
2 Research and Development Center, China Tobacco Chuanyu Industrial Corporation, Chengdu, P.R. China

We compared stomatal ammonia compensation point (χs) and its metabolic regulation in tobacco (Nicotiana tabacum) leaves of a quick-leaf-senescence phenotype ZY90 and a slow-leaf-senescence phenotype NC89. Compared with NC89, ZY90 had significantly higher χs values between 40 and 60 d after leaf sprouting in spite of its lower nitrogen content. During the same time, a steeper decline in glutamine synthetase activity was detected in ZY90 leaves, simultaneously with a steep increase in χs. These results suggested that the quick leaf senescence phenotype exhibited high NH3 emission potential due to efficient nitrogen recycling and remobilization, and glutamine synthetase played a key role in regulating χs in ZY90.

Keywords: glutamine synthetase; nitrogen content; quick- and slow-leaf-senescence phenotypes; tobacco
Subjects: amonium compensation point; senescence; nitrogen content; glutamine synthetase; apoplast; proteins; glutamine dehydrogenase

Received: March 2, 2011; Accepted: December 5, 2011; Published: December 1, 2012  Show citation

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Duan, W.J., Yang, T.Z., Dai, Y., Li, D.L., Zhang, X.Q., Liu, H.B., Li, N., & Wang, C.G. (2012). Stomatal NH3 compensation point and its metabolic regulation in senescence phenotypes of Nicotiana tabacum. Biologia plantarum, 56(4), 771-774. doi: 10.1007/s10535-012-0141-z
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