biologia plantarum

International journal on Plant Life established by Bohumil Nìmec in 1959

Biologia plantarum 56:789-792, 2012 | DOI: 10.1007/s10535-012-0134-y

New polymorphic microsatellite loci for Theobroma cacao: isolation and characterization of microsatellites from enriched genomic libraries

E. S. L. Santos1,2, C. B. M. Cerqueira-Silva1,2, G. M. Mori2, D. Ahnert3, R. X. Corrêa3, A. P. Souza2,4,*
1 Departamento de Estudos Básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, Itapetinga, Bahia, Brazil
2 Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil
3 Centro de Biotecnologia e Genética, Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Ilhéus, Bahia, Brazil
4 Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, Brazil

Seventeen polymorphic microsatellite markers were isolated from enriched genomic libraries for Theobroma cacao, providing additional tools for studying the genetic diversity and map saturation of this species. These markers were characterized in 32 accessions of the T. cacao germplasm collection from the Centro de Pesquisas do Cacau. The number of alleles at each locus varied from 2 to 8, with an average of 4.41 alleles per locus. The polymorphism information content varied from 0.060 to 0.695, with an average of 0.333. The markers characterized in this study will be employed in map saturation studies and diversity assessments of cacao genotypes.

Keywords: cacao; genetic diversity; molecular markers; PCR
Subjects: genetic diversity; microsatellite markers; PCR

Received: July 14, 2011; Accepted: December 20, 2011; Published: December 1, 2012  Show citation

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Santos, E.S.L., Cerqueira-Silva, C.B.M., Mori, G.M., Ahnert, D., Corrêa, R.X., & Souza, A.P. (2012). New polymorphic microsatellite loci for Theobroma cacao: isolation and characterization of microsatellites from enriched genomic libraries. Biologia plantarum, 56(4), 789-792. doi: 10.1007/s10535-012-0134-y
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