biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:26-32, 2013 | DOI: 10.1007/s10535-012-0146-7

Isolation and characterization of a novel transcriptional repressor GmERF6 from soybean

Y. Zhai1, J. -W. Li1, X. -W. Li2, T. -T. Lei1, F. Yan1, Y. Zhao3, Y. -J. Li1, L. -T. Su1, Y. Wang1,*, Q. -Y. Wang1,*
1 College of Plant Science, Jilin University, Changchun, Jilin, P.R. China
2 Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun, Jilin, P.R. China
3 College of Life Science and Agroforestry, Qiqihaer University, Qiqihaer, Heilongjiang, P.R. China

A new ethylene response factor (ERF), GmERF6, was isolated from soybean. Protein sequence alignment of GmERF6 revealed an AP2/ERF domain, two putative nuclear localization signals (NLSs) and an ERF-associated amphiphilic repression (EAR) motif. Real-time quantitative PCR analysis revealed that the expression of GmERF6 was differentially induced in soybean seedlings by drought, salt, cold, salicylic acid, ethylene, abscisic acid and methyl jasmonate. Transient expression experiments demonstrated that GmERF6 functions as a transcriptional repressor to downregulate the transcriptional levels of the reporter gene and repress the activated ability of other transcriptional activator. Transgenic Arabidopsis lines constitutively expressing GmERF6 showed an increased tolerance to drought compared to wild-type plants.

Keywords: Arabidopsis thaliana; drought tolerance; ethylene response factor; expression pattern; Glycine max; transcriptional regulation
Subjects: transcriptional repressor; soybean; drought tolerance; ethylene response factor; expression pattern

Received: September 16, 2011; Accepted: February 16, 2012; Published: March 1, 2013  Show citation

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Zhai, Y., Li, J.-W., Li, X.-W., Lei, T.-T., Yan, F., Zhao, Y., ... Wang, Q.-Y. (2013). Isolation and characterization of a novel transcriptional repressor GmERF6 from soybean. Biologia plantarum, 57(1), 26-32. doi: 10.1007/s10535-012-0146-7
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