biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:41-48, 2013 | DOI: 10.1007/s10535-012-0254-4

The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro

O. M. Tun1, A. Lipsky2, T. Luzzatto Knaan1, Z. Kerem1, I. Yedidia2,*
1 Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
2 Department of Plant Sciences, Agricultural Research Organization, The Volcani Center, Bet-Dagan, Israel

Benzothiadiazole (BTH) is a structural analogue of salicylic acid (SA) which is widely recognized for its role in elicitation of systemic acquired resistance in a broad range of plant species. Here, BTH was applied to cell cultures of the bulbous ornamental plants Ornithogalum dubium and O. thyrsoides, showing a strong effect on rates of differentiation and morphogenesis. Morphogenic cell clusters in liquid Murashige and Skoog (MS) medium containing 1-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) were used for all treatments. The calluses were washed thoroughly and activated with increasing concentrations of BTH. Following the induction, calli were grown on a solid MS medium without growth regulators (MS) or on a comparable media with NAA and BAP (M-206). The calli treated with BTH displayed a dose dependent increase in formation of meristematic centres followed by enhanced shoot formation compared to controls. Microscopic analyses revealed increased differentiation to cell organelles and a strengthening of the cell wall. A stronger response to BTH was observed in MS than in M-206 medium. A similar effect on calli differentiation was obtained by three weeks darkness followed by light exposure. The dark/light positive effect on differentiation was further augmented by BTH in a synergistic fashion. It is suggested that BTH enhances the rates of morphogenesis in Ornithogalum cultures by triggering a plant regulator-like activity.

Keywords: benzothiadiazole; 6-benzylaminopurine; micropropagation; morphogenesis; 1-naphthaleneacetic acid
Subjects: benzothiadiazole; in vitro; in vitro culture; auxin; cytokinin; morphogenesis

Received: June 7, 2011; Accepted: February 28, 2012; Published: March 1, 2013  Show citation

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Tun, O.M., Lipsky, A., Luzzatto Knaan, T., Kerem, Z., & Yedidia, I. (2013). The plant activator BTH promotes Ornithogalum dubium and O. thyrsoides differentiation and regeneration in vitro. Biologia plantarum, 57(1), 41-48. doi: 10.1007/s10535-012-0254-4
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