biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:79-84, 2013 | DOI: 10.1007/s10535-012-0138-7

Molecular mapping of QTLs for wheat flag leaf senescence under water-stress

M. N. Barakat1,*, L. E. Wahba2, S. I. Milad2
1 College of Food Sciences and Agriculture, King Saud University, Riyadh, Saudi Arabia
2 Faculty of Agriculture, Alexandria University, Alexandria, Egypt

A segregating population from the cross between drought sensitive (Variant-2) and drought tolerant (Cham-6) genotypes was made to identify molecular markers linked to wheat (Triticum aestivum L.) flag leaf senescence under water-stress. From 38 random amplified polymorphic DNA (RAPD) primers, 25 inter-simple sequence repeat (ISSR) primers and 46 simple sequence repeat (SRR) primers, tested for polymorphism among parental genotypes and F2 population. Quantitative trait locus (QTL) for flag leaf senescence was associated with 1 RAPD marker (Pr9), 4 ISSR markers (Pr8, AD5, AD2 and AD3), and 1 SSR marker (Xgwm382) and explained 44, 50, 35, 31, 22 and 73 % phenotypic variation, respectively. The genetic distance between flag leaf senescence gene and Pr9 was 10.0 cM (LOD score 22.9). The markers Pr8, AD5, AD2 and AD3 had genetic distances of 10.5, 14.6, 15.6 and 18.1 cM, respectively (LOD scores 22.6, 17.8, 17.5 and 14.6). The genetic distance between Xgwm382 was 3.9 cM (LOD score 33.8). Therefore, the RAPD, ISSR and SSR markers linked to the QTL for the drought-induced flag leaf senescence can be further used in breeding for drought tolerance in wheat.

Keywords: drought tolerance; ISSR; RAPD; SSR; Triticum aestivum
Subjects: molecular mapping; quantitative trait loci; leaf senescence; wheat; water-stress; drought tolerance; RAPD; ISSR; SSR

Received: December 11, 2010; Accepted: February 7, 2012; Published: March 1, 2013  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Barakat, M.N., Wahba, L.E., & Milad, S.I. (2013). Molecular mapping of QTLs for wheat flag leaf senescence under water-stress. Biologia plantarum, 57(1), 79-84. doi: 10.1007/s10535-012-0138-7
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Altinkut, A., Gozukirmizi, N.: Search for microsatellite markers associated with water-stress tolerance in wheat through bulked segregant analysis. - Mol. Biotechnol. 23: 97-106, 2003. Go to original source...
    2. Baenziger, M., Edmeades, G.O., Lafitte, H.R.: Selection for drought tolerance increases maize yields across a range of nitrogen levels. - Crop Sci. 39: 1035-1040, 1999. Go to original source...
    3. Barakat, M.N., Al-Doss, A.A., Moustafa, K.A., Ahmed, E.I., Elshafei, A.A.: Morphological and molecular characterization of Saudi wheat genotypes under drought stress. - J. Food Agr. Environ. 8: 220-228, 2010.
    4. Barakat, M.N., Milad, S.I., Imbaby, I.A.: Field evaluation for rust diseases and RAPD analysis for somaclonal variant lines in wheat. - Alexandria J. agr. Res. 50: 11-24, 2005.
    5. Beavis, W.D., Smith, O.S., Grant, D., Fincher, R.: Identification of quantitative trait loci using a small sample of top-crossed and F4 progeny from maize. - Crop Sci. 34: 882-896, 1994. Go to original source...
    6. Blum, A. (ed.): Plant Breeding for Stress Environments. - CRC Press, Boca Raton 1988.
    7. Bradley, S.R., Scott, R.K., Wright, C.E. (ed.): Physiology in the Production and Improvement of Cereals. - Home-Grown Cereals Authority, London 1990.
    8. Chandler, J.M.: Current molecular understanding of the genetically programmed process of leaf senescence. - Physiol. Plant. 113: 1-8, 2001. Go to original source...
    9. Evans, L.T. (ed.): Crop Evolution, Adaptation, and Yield. - Cambridge University Press, Cambridge 1993.
    10. Forster, B.P., Ellis, R.P., Thomas, W.T.: The development and application of molecular markers for abiotic stress tolerance in barley. - J. exp. Bot. 51: 19-27, 2000. Go to original source...
    11. Govindaraj, P., Arumugachamy, S., Maheswaran, M.: Bulked segregant analysis to detect main effect QTL associated with grain quality parameters in Basmati 370/ASD16 cross in rice (Oryza sativa L.) using SSR markers. - Euphytica 144: 61-68, 2005. Go to original source...
    12. Hafsi, M., Mechmeche, W., Bouamama, L., Djekoune, A., Zaharieva, M., Monneveux, P.: Flag leaf senescence, as evaluated by numerical image analysis, and its relationship with yield under drought in durum wheat. - J. Agron. Crop Sci. 185: 275-280, 2000. Go to original source...
    13. Harris, K., Subudhi, P.K., Borrel, A., Jordan, D., Rosenow, D., Nguyen, H., Klein, P., Klein, R., Mullet, J.: Sorghum staygreen QTL individually reduce post-flowering droughtinduced leaf senescence. - J. exp. Bot. 58: 327-338, 2007. Go to original source...
    14. Hu, X.J., Zhang, Z.B., Xu, Z.Y., Fu, Z.Y., Hu, S.B., Song, W.Y.: Multifunctional genes: the cross-talk among the regulation networks of abiotic stress responses. - Biol. Plant. 54: 695-702, 2010. Go to original source...
    15. Kebede, H., Subudhi, P.K., Rosenow, D.T., Nguyen, H.T.: Quantitative trait loci influencing drought tolerance in grain sorghum (Sorghum bicolor L. Moench). - Theor. appl. Genet. 103: 266-276, 2001. Go to original source...
    16. Lawson, W.R., Henry, R.J., Kochman, J.K., Kong, G.A.: Genetic diversity in sunflower (Helianthus annus L.) as revealed by random amplified polymorphic DNA analysis. - Aust. J. agr. Res. 45: 1319-1327, 1994. Go to original source...
    17. Luo, L.J., Zhang, Q.F.: The status and strategy on drought resistance of rice (Oryza sativa L.). - Chinese J. Rice Sci. 15: 209-214, 2001.
    18. Mackay, I.J., Caligari, P.D.S.: Efficiencies in F2 and backcross generations for bulked segregant analysis using dominant markers. - Crop Sci. 40: 626-630, 2000. Go to original source...
    19. Michelmore, R.W., Paran, I., Kesseli, R.V.: Identification of markers linked to disease-resistance genes by bulked segregant anaylsis: A rapid method to detect markers in specific genomics regions by using segregating populations. - Proc. nat. Acad. Sci. USA 88: 9828-9852, 1991. Go to original source...
    20. Nelson, J.C.: QGENE: software for marker-based genomic analysis and breeding. - Mol. Breed. 3: 239-245, 1997. Go to original source...
    21. Passioura, J.B.: Environmental biology and crop improvement. - Funct. Plant Biol. 29: 537-546, 2002. Go to original source...
    22. Podlich, D.W., Winkler, C.R., Cooper, M.: Mapping as you go: an effective approach for marker-assisted selection of complex traits. - Crop Sci. 44: 1560-1571, 2004. Go to original source...
    23. Rajaram, S., Braun, H-J., Van Ginkel, M.: CIMMYT's approach to breed for drought tolerance. - Euphytica 92: 147-153, 1996. Go to original source...
    24. Röeder, M.S., Korzun, V., Gill, B.S., Ganal, M.W.: The physical mapping of microsatellite markers in wheat. - Genome 41: 278-283, 1998. Go to original source...
    25. Rosenow, D.T., Clark, L.E.: Drought tolerance in sorghum. - In: Loden, H.D., Wilkinson, D. (ed.): Proceedings of the 36th Annual Corn and Sorghum Industry Research Conference. Pp. 18-31. Chicago 1981.
    26. Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W.: Ribosomal DNA spacer-length polymorphism in barley: Mendelian inheritance, chromosomal location, and population dynamics. - Proc. nat. Acad. Sci. USA 81: 8014-8018, 1984. Go to original source...
    27. Smith, M.: CROPWAT: Manual and Guidelines. - FAO of UN, Rome 1991.
    28. Sweeney, P.M., Danneberger, T.K.: Random amplified polymorphic DNA in perennial ryegrass: a comparison of bulk samples vs. individuals. - Hort. Sci. 29: 624-626, 1994. Go to original source...
    29. Tripathy, J.N., Zhang, J., Robin, S., Nguyen, H.T.: QTLs for cell-membrane stability mapped in rice (Oryza sativa L.) under drought stress. - Theor. appl. Genet. 100: 1197-1202, 2000. Go to original source...
    30. Tuberosa, R., Salvi, S.: Genomics-based approaches to improve drought tolerance of crops. - Trends Plant Sci. 11: 405-412, 2007. Go to original source...
    31. Verma, V., Foulkes, M.J., Caligari, P., Bradley, S.R., Snape, J.: Mapping QTLs for flag leaf senescence as a yield determinant in winter wheat under optimal and droughted environments. - Euphytica 135: 255-263, 2004. Go to original source...
    32. Visser, B.: Technical aspects of drought tolerance. - Biotechnol. Dev. Monitor. 18: 5, 1994.
    33. Xu, B.Y., Shen, Z. T., Chen, Y.: Molecular mapping for quantitative trait loci controlling rice yield. - Acta genet. sin. 22: 46-52, 1995.
    34. Zheng, X.Y., Wang, Y.J., Song, S.H., Li, L., Yu, S.C.: [Identification of heat tolerance linked molecular markers of Chinese cabbage Brassica campestris ssp. pekinensis.] - Agr. Sci. China 1: 309-313, 2002. [In Chinese]

    Return to the content