biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:105-112, 2013 | DOI: 10.1007/s10535-012-0237-5

Accumulation of WCS120 and DHN5 proteins in differently frost-tolerant wheat and barley cultivars grown under a broad temperature scale

K. Kosová1,*, P. Vítámvás1, P. Prášilová1, I. T. Prášil1
1 Department of Genetics and Plant Breeding, Crop Research Institute, Prague 6 - Ruzyně, Czech Republic

Proteins WCS120 and DHN5 are known as the major cold-inducible dehydrins in wheat and barley plants, respectively. WCS120 and DHN5 relative accumulation increased exponentially along with a growth temperature decline in the range from optimum to cold temperatures. Even at optimum growth temperatures, the most frost-tolerant wheat and barley cultivars can be distinguished from the remaining ones according to dehydrin relative accumulation. The highly tolerant wheat and barley cultivars started accumulating dehydrins at higher growth temperatures and reached higher dehydrin amounts than the less tolerant ones. Statistically significant correlations between lethal temperature for 50 % of the samples (LT50) and dehydrin relative accumulation have been found at all growth temperatures (5, 10, 15 and 20 °C) for WCS120 in wheats and at 5 and 10 °C for DHN5 in barleys. Analogous relationships between dehydrin relative accumulation at different growth temperatures and plant acquired frost tolerance have been proved for wheat WCS120 and barley DHN5.

Keywords: cold acclimation; dehydrins; lethal temperature; Hordeum vulgare; Triticum aestivum
Subjects: cold acclimation; frost resistance; temperature low; dehydrins; wheat; barley; temperature lethal

Received: November 24, 2011; Accepted: March 13, 2012; Published: March 1, 2013  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Kosová, K., Vítámvás, P., Prášilová, P., & Prášil, I.T. (2013). Accumulation of WCS120 and DHN5 proteins in differently frost-tolerant wheat and barley cultivars grown under a broad temperature scale. Biologia plantarum, 57(1), 105-112. doi: 10.1007/s10535-012-0237-5
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Badawi, M., Danyluk, J., Boucho, B., Houde, M., Sarhan, F.: The CBF gene family in hexaploid wheat and its relationship to the phylogenetic complexity of cereal CBFs. - Mol. Genet. Genomics 277: 533-554, 2007. Go to original source...
    2. Bradford, M.M.: A rapid and sensitive method for the quantities of protein by an improved protein-dye binding assay. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. Bravo, L.A., Close, T.J., Corcuera, L.J., Guy, C.L.: Characterization of an 80-kDa dehydrin-like protein in barley responsive to cold acclimation. - Physiol. Plant. 106: 177-183, 1999. Go to original source...
    4. Bravo, L.A., Gallardo, J., Navarrete, A., Olave, N., Martínez, J., Alberdi, M., Close, T.J., Corcuera, L.J.: Cryoprotective activity of a cold-induced dehydrin purified from barley. - Physiol. Plant. 118: 262-269, 2003. Go to original source...
    5. Campoli, C., Matus-Cádiz, M.A., Pozniak, C.J., Cattivelli, L., Fowler, D.B.: Comparative expression of Cbf genes in the Triticeae under different acclimation induction temperatures. - Mol. Genet. Genomics 282: 141-152, 2009. Go to original source...
    6. Choi, D.W., Zhu, B., Close, T.J.: The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allele types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv. Dicktoo. - Theor. appl. Genet. 98: 1234-1247, 1999. Go to original source...
    7. Close, T.J.: Dehydrins: A commonalty in the response of plants to dehydration and low temperature. - Physiol. Plant. 100: 291-296, 1997. Go to original source...
    8. Close, T.J., Fenton, R.D., Moonan, F.: A view of plant dehydrins using antibodies specific to the carboxy-terminal peptide. - Plant mol. Biol. 23: 279-286, 1993. Go to original source...
    9. Crosatti, C., Soncini, C., Stanca, A.M., Cattivelli, L.: The accumulation of a cold-regulated chloroplastic protein is light-dependent. - Planta 196: 458-463, 1995. Go to original source...
    10. Danyluk, J., Kane, N.A., Breton, G., Limin, A.E., Fowler, D.B., Sarhan, F.: TaVRT-1, a putative transcription factor associated with vegetative to reproductive transition in cereals. - Plant Physiol. 132: 1849-1860, 2003. Go to original source...
    11. Fowler, D.B.: Cold acclimation threshold induction temperatures in cereals. - Crop Sci. 48: 1147-1154, 2008. Go to original source...
    12. Fowler, D.B., Limin, A.E., Ritchie, J.T.: Low-temperature tolerance in cereals: model and genetic interpretation. - Crop Sci. 39: 626-633, 1999. Go to original source...
    13. Fowler, D.B., Breton, G., Limin, A.E., Mahfoozi, S., Sarhan, F.: Photoperiod and temperature interactions regulate low-temperature-induced gene expression in barley. - Plant Physiol. 127: 1676-1681, 2001. Go to original source...
    14. Galiba, G., Vágújfalvi, A., Li, C., Soltész, A., Dubcovsky, J.: Regulatory genes involved in the determination of frost tolerance in temperate cereals. - Plant Sci. 176: 12-19, 2009. Go to original source...
    15. Ganeshan, S., Vítámvás, P., Fowler, D.B., Chibbar, R.N.: Quantitative expression analysis of selected COR genes reveals their differential expression in leaf and crown tissues of wheat (Triticum aestivum L.) during an extended low temperature acclimation regimen. - J. exp. Bot. 59: 2393-2402, 2008. Go to original source...
    16. Holková, L., Prášil, I.T., Bradáčová, M., Vítámvás, P., Chloupek, O.: Screening for frost tolerance in wheat using the expression of dehydrin genes Wcs120 and Wdhn13 at 17 °C. - Plant Breed. 128: 420-422, 2009. Go to original source...
    17. Houde, M., Dhindsa, R.S., Sarhan, F.: A molecular marker to select for freezing tolerance in Gramineae. - Mol. gen. Genet. 234: 43-48, 1992. Go to original source...
    18. Houde, M., Daniel, C., Lachapelle, M., Allard, F., Laliberté, S., Sarhan, F.: Immunolocalization of freezing-tolerance-associated proteins in the cytoplasm and nucleoplasm of wheat crown tissues. - Plant J. 8: 583-593, 1995. Go to original source...
    19. Janáček, J., Prášil, I.: Quantification of plant frost injury by nonlinear fitting of an S-shaped function. - Cryo-Letters 12: 47-52, 1991.
    20. Klíma, M., Vítámvás, P., Zelenková, S., Vyvadilová, M., Prášil, I.T.: Dehydrin and proline content in Brassica napus and B. carinata under cold stress at two irradiances. - Biol. Plant. 56: 157-161, 2012. Go to original source...
    21. Knox, A.K., Li, C., Vágújfalvi, A., Galiba, G., Stockinger, E.J., Dubcovsky, J.: Identification of candidate CBF genes for the frost tolerance locus Fr-A m 2 in Triticum monococcum. - Plant mol. Biol. 67: 257-270, 2008. Go to original source...
    22. Knox, A.K., Dhillon, T., Cheng, H., Tondelli, A., Pecchioni N., Stockinger, E.J.: CBF gene copy number variation at Frost-resistance-2 is associated with levels of freezing tolerance in temperate-climate cereals. - Theor. appl. Genet. 121: 21-35, 2010. Go to original source...
    23. Kosová, K., Vítámvás, P., Prášil, I.T.: The role of dehydrins in plant response to cold. - Biol. Plant. 51: 601-617, 2007. Go to original source...
    24. Kosová K., Holková, L., Prášil, I.T., Prášilová, P., Bradáčová, M., Vítámvás, P., Čapková, V.: Expression of dehydrin 5 during the development of frost tolerance in barley (Hordeum vulgare). - J. plant Physiol. 165: 1142-1151, 2008. Go to original source...
    25. Kosová K., Prášil I.T., Vítámvás P.: Role of dehydrins in plant stress response. - In: Pessarakli M. (ed.): Handbook of Plant and Crop Stress. 3rd Ed. Pp. 239-285. CRC Press, Taylor & Francis, Boca Raton 2010. Go to original source...
    26. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. - Nature 277: 680-685, 1970. Go to original source...
    27. Miller, A.K., Galiba, G., Dubcovsky, J.: A cluster of 11 CBF transcription factors is located at the frost tolerance locus Fr-A m 2 in Triticum monococcum. - Mol. Genet. Genomics 275: 193-203, 2006. Go to original source...
    28. Prášil, I.T., Prášilová, P., Mařík, P.: Comparative study of direct and indirect evaluations of frost tolerance in barley. - Field Crops Res. 102: 1-8, 2007. Go to original source...
    29. Rorat, T.: Plant dehydrins - tissue location, structure and functions. - Cell. mol. Biol. Lett. 11: 536-556, 2006. Go to original source...
    30. Sarhan, F., Ouellet, F., Vazquez-Tello, A.: The wheat wcs120 gene family. A useful model to understand the molecular genetics of freezing tolerance in cereals. - Physiol. Plant. 101: 439-445, 1997. Go to original source...
    31. Stockinger, E.J., Skinner, J.S., Gardner, K.G., Francia, E., Pecchioni, N.: Expression levels of barley Cbf genes at the Frost resistance-H2 locus are dependent upon alleles at Fr-H1 and Fr-H2. - Plant J. 51: 308-321, 2007. Go to original source...
    32. Thomashow, M.F.: Plant cold acclimation: freezing tolerance genes and regulatory mechanisms. - Annu. Rev. plant Physiol. plant mol. Biol. 50: 571-599, 1999. Go to original source...
    33. Tommasini, L., Svensson, J.T., Rodriguez, E.M., Wahid, A., Malatrasi, M., Kato, K., Wanamaker, S., Resnik, J., Close, T.J.: Dehydrin gene expression provides an indicator of low temperature and drought stress: transcriptome-based analysis of barley (Hordeum vulgare L.). - Funct. integr. Genomics 8: 387-405, 2008. Go to original source...
    34. Tondelli, A., Francia, E., Barabaschi, D., Pasquariello, M., Pecchioni, N.: Inside the CBF locus in Poaceae. - Plant Sci. 180: 39-45, 2011. Go to original source...
    35. Vágújfalvi, A., Crosatti, C., Galiba, G., Dubcovsky, J., Cattivelli, L.: Two loci on wheat chromosome 5A regulate the differential cold-dependent expression of the cor14b gene in frost-tolerant and frost-sensitive genotypes. - Mol. gen. Genet. 263: 194-200, 2000. Go to original source...
    36. Vágújfalvi, A., Galiba, G., Cattivelli, L., Dubcovsky, J.: 2003: The cold-regulated transcriptional activator Cbf3 is linked to the frost-tolerance locus Fr-A2 on wheat chromosome 5A. - Mol. gen. Genomics 269: 60-67, 2003. Go to original source...
    37. Van Zee, K., Chen, F.Q., Hayes, P.M., Close, T.J., Chen, T.H.H.: Cold-specific induction of a dehydrin gene family member in barley. - Plant Physiol. 108: 1233-1239, 1995. Go to original source...
    38. Vítámvás, P., Saalbach, G., Prášil, I.T., Čapková, V., Opatrná, J., Jahoor, A.: WCS120 protein family and proteins soluble upon boiling in cold-acclimated winter wheat. - J. plant Physiol. 164: 1197-1207, 2007. Go to original source...
    39. Vítámvás, P., Prášil, I.T.: WCS120 protein family and frost tolerance during cold acclimation, deacclimation and reacclimation of winter wheat. - Plant Physiol. Biochem. 46: 970-976, 2008. Go to original source...
    40. Vítámvás, P., Kosová, K., Prášilová, P., Prášil, I.T.: Accumulation of WCS120 protein in wheat cultivars grown at 9°C or 17°C in relation to their winter survival. - Plant Breed. 129: 611-616, 2010. Go to original source...
    41. Zhu, B., Choi, D.-W., Fenton, R., Close, T.J.: Expression of the barley dehydrin multigene family and the development of freezing tolerance. - Mol. gen. Genet. 264: 145-153, 2000. Go to original source...

    Return to the content