biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:113-120, 2013 | DOI: 10.1007/s10535-012-0259-z

Characterization of phospholipase D from Chorispora bungeana callus in response to freezing stress

N. Yang1,2, X. L. Yue1, H. Zhang1, G. F. Wu2, F. X. Ding2, T. G. Zhang2, L. Z. An1,*
1 Key Laboratory of Arid and Pasture Agroecology of Ministry of Education, School of Life Science, Lanzhou University, Lanzhou, P.R. China
2 School of Life Science, Northwest Normal University, Lanzhou, P.R. China

The influence of freezing on phospholipase D (PLD) was studied in Chorispora bungeana Fisch. & C.A. Mey., which is a naturally cold-tolerant species. During the freezing treatment (-4 °C), PLD activities in both microsomal and mitochondrial membranes increased at day 3, remained at a high level at day 6 and then declined to a moderate level. The RT-PCR analyses showed that PLD activity partially corresponded to the CbPLD gene transcript level. The freezing treatment resulted in increases in the K m and V max for microsomal and mitochondrial PLD, respectively. Freezing injury, as measured by electrolyte leakage and malondialdehyde content, peaked at day 6 and then gradually decreased. Alleviation of freezing injury was related to a decreased content of membrane-associated Ca2+. We suggest that the specific mechanism of cold resistance of C. bungeana is linked with PLD.

Keywords: calcium; electrolyte leakage; malondialdehyde; microsomal and mitochondrial membranes; RT-PCR
Subjects: phospholipase D; freezing stress; temperature low; calcium; electrolyte leakage; mitochondrial membranes; microsomal membranes; in vitro culture

Received: February 17, 2011; Accepted: May 16, 2011; Published: March 1, 2013  Show citation

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Yang, N., Yue, X.L., Zhang, H., Wu, G.F., Ding, F.X., Zhang, T.G., & An, L.Z. (2013). Characterization of phospholipase D from Chorispora bungeana callus in response to freezing stress. Biologia plantarum, 57(1), 113-120. doi: 10.1007/s10535-012-0259-z
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