biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:474-480, 2013 | DOI: 10.1007/s10535-012-0300-2

Structural, physiological, and biochemical profiling of tea plants under zinc stress

M. Mukhopadhyay1,3, A. Das1, P. Subba1, P. Bantawa1, B. Sarkar2, P. Ghosh3, T. K. Mondal1,4,*
1 Biotechnology Laboratory, Faculty of Horticulture, Uttar Banga Krishi Viswavidyalaya, Cooch Behar, India
2 Department of Chemistry, Faculty of Science, North Bengal University, Darjeeling, India
3 Department of Botany, University of Kalyani, Kalyani, Nadia, India
4 National Research Center on DNA Fingerprinting, National Bureau of Plant Genetic Resources, Pusa, India

Zinc is the most widespread deficient micronutrient in the tea growing soils of India which affects growth of the plants. In order to investigate the structural, physiological, and biochemical changes under Zn stress (i.e. both deficient and excess supply) of tea [Camellia sinensis (L.) O. Kuntze cv. T-78] plants, we treated young plants with ZnSO4 at 0 (deficiency), 0.3, 3 (optimum), and 30 μM (toxic) concentrations for 8 weeks. Zn deficiency and excess resulted in considerable decrease in shoot and root fresh and dry masses, and transmission electron microscopy (TEM) revealed disorganization of some cellular organelles. Further, Zn-stress decreased net photosynthetic rate (PN), transpiration rate (E), stomatal conductance (gs), and content of chlorophylls a and b. On the other hand, content of superoxide anion, malondialdehyde, hydrogen peroxide, and phenols, and electrolyte leakage were elevated in stressed plants. The activities of ascorbate peroxidase, catalase, superoxide dismutase, and peroxidase as well as expression of respective genes were up-regulated under Zn-stress. Nevertheless, antioxidant system as a whole did not afford sufficient protection against oxidative damage.

Keywords: antioxidative enzymes; Camellia sinensis; net photosynthetic rate; reactive oxygen species; RT PCR; transpiration rate
Subjects: zinc; net photosynthetic rate; reactive oxygen species; transpiration rate; ultrastructure; chlorophyll; carotenoids; hydrogen peroxide; malondialdehyde; electrolyte leakage; sugars; starch; phrnols; phenols; ascorbate peroxidase; catalase; peroxidase; superoxide dismutase; tea

Received: April 11, 2012; Accepted: October 15, 2012; Published: September 1, 2013  Show citation

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Mukhopadhyay, M., Das, A., Subba, P., Bantawa, P., Sarkar, B., Ghosh, P., & Mondal, T.K. (2013). Structural, physiological, and biochemical profiling of tea plants under zinc stress. Biologia plantarum, 57(3), 474-480. doi: 10.1007/s10535-012-0300-2
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