biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:571-575, 2013 | DOI: 10.1007/s10535-013-0308-2

Early transcription of defence-related genes in Platanus × acerifolia leaves following treatment with cerato-platanin

I. Baccelli1,*, A. Scala1, L. Pazzagli2, R. Bernardi3
1 Dipartimento di Scienze delle Produzioni Agroalimentari e dell'Ambiente, Università di Firenze, Firenze, Italy
2 Dipartimento di Scienze Biomediche Cliniche e Sperimentali, Università di Firenze, Firenze, Italy
3 Dipartimento di Scienze Agrarie, Alimentari e Agro-ambientali, Università di Pisa, Pisa, Italy

The protein elicitor cerato-platanin (CP) is known to induce defence-related responses in various plants. Some of these responses occur very quickly. In the present work, transcriptional changes caused by CP in leaves from Platanus × acerifolia (Aiton) Willd. were studied. With a cDNA microarray, 131 differentially regulated transcripts were identified as responsive to CP after 24 h of treatment. Eighty-six of these were cold-or ozone-modulated transcripts, thus revealing a significant overlap between genes responsive to CP and to cold/ozone stress. The transcriptional changes caused by CP were compared with the CP-orthologous protein Pop1 in a time-course analysis performed after 3, 6, 12, and 24 h of treatment by real-time RT-PCR on five defence-related genes. Despite some differences, CP and Pop1 were both able to induce early transcriptional changes (WRKY was overexpressed after only 3 h) confirming that pathogenassociated molecular patterns (PAMPs) act very quickly on gene transcription.

Keywords: cerato-populin; cold stress; ozone; plane tree; poplar; RT-PCR
Subjects: defence-related genes; Plant-pathogen interactions; cerato-platanin; cerato-populin; ozone; temperature - low; gene expression; plane tree

Received: February 28, 2012; Accepted: November 2, 2012; Published: September 1, 2013  Show citation

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Baccelli, I., Scala, A., Pazzagli, L., & Bernardi, R. (2013). Early transcription of defence-related genes in Platanus × acerifolia leaves following treatment with cerato-platanin. Biologia plantarum, 57(3), 571-575. doi: 10.1007/s10535-013-0308-2
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