biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:576-580, 2013 | DOI: 10.1007/s10535-013-0319-z

Effects of paclobutrazol in vitro on transplanting efficiency and root tip development of Dendrobium nobile

Z. Z. Wen1,2, Y. Lin1,2,3, Y. Q. Liu1,2, M. Wang1, Y. Q. Wang1,2, W. Liu1,2,*
1 College of Life Sciences, South China Agricultural University, Guangzhou, P.R. China
2 Key Laboratory of Plant Functional Genomics and Biotechnology of Education Department, Guangdong Province, Guangzhou, P.R. China
3 Dongguan Flower Research Institute, Dongguan, P.R. China

The effects of paclobutrazol (PBZ) on the ex vitro transfer efficiency of in vitro grown Dendrobium nobile seedlings were investigated. The survival percentage was increased by 41.6 % with 0.8 mg dm-3 PBZ treatment compared to controls. The PBZ-treated D. nobile plants were shorter than control plants. Morphological and anatomical analyses showed that root diameter, especially at the root apex, became larger after treatment with PBZ which is consistent with the increases in cortical cell sizes and row numbers. In addition, the first observations of thickened velamen of the PBZ-treated seedling were made in the present study. The activities of cellulase and indole acetic acid oxidase increased in PBZ-treated plantlets, whereas that of cell wall-associated peroxidase declined compared to controls. The content of endogenous gibberellic acid and iso-pentenyladenosine of root tips changed little by PBZ but that of indole-3-acetic acid decreased by 53 %. These results indicated that PBZ could improve the transfer efficiency of D. nobile from in vitro culture to pots due to its effects on root development.

Keywords: gibberellic acid; indole-3-acetic acid; iso-pentenyladenosine; micropropagation; root anatomy and morphology
Subjects: paclobutrazol; in vitro culture; ex vitro transfer; auxin; gibberellic acid; cytokinins; root anatomy; cellulase; peroxidase; orchid

Received: July 18, 2012; Accepted: January 16, 2013; Published: September 1, 2013  Show citation

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Wen, Z.Z., Lin, Y., Liu, Y.Q., Wang, M., Wang, Y.Q., & Liu, W. (2013). Effects of paclobutrazol in vitro on transplanting efficiency and root tip development of Dendrobium nobile. Biologia plantarum, 57(3), 576-580. doi: 10.1007/s10535-013-0319-z
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