biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:646-654, 2013 | DOI: 10.1007/s10535-013-0355-8

Gene expression of the oil palm transcription factor EgAP2-1 during fruit ripening and in response to ethylene and ABA treatments

V. Omidvar1,2, S. N. A. Abdullah1,2,*, M. Ebrahimi1, C. L. Ho2,3, M. Mahmood3
1 Department of Agriculture Technology, Faculty of Agriculture, University Putra Malaysia, Selangor, Malaysia
2 Laboratory of Plantation Crops, Institute of Tropical Agriculture, University Putra Malaysia, Selangor, Malaysia
3 Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Selangor, Malaysia

A cDNA encoding an ERE-binding protein (EgAP2-1) was isolated from the oil palm fruit mesocarp treated with ethylene using yeast one-hybrid assay. EgAP2-1 belongs to the AP2 subfamily of the APETALA2/ethylene-responsive factor (AP2/ERF) proteins and contains two highly conserved AP2/EREBP DNA-binding domains (DNA-BD). Sequence comparison of EgAP2-1 with other AP2 proteins revealed high conservation of the two AP2/EREBP domains and linker region among these proteins. Its protein was localized to the nucleus of onion epidermis cells and showed ERE-specific binding, transcriptional activation, and transactivation properties in yeast and in vitro. Its mRNA was highly expressed in oil palm mesocarp with elevated levels in ripening fruits but not in leaves and roots. EgAP2-1 was induced in mesocarp in response to ethylene and abscisic acid but not other hormonal stimuli, including methyl jasmonate and salicylic acid, and abiotic stresses including drought, cold, and high-salinity. Our results demonstrate a link between the regulation of EgAP2-1 expression and ethylene- and/or ABA-coordinated control of the fruit ripening and suggest a regulatory role for EgAP2-1 during fruit ripening and development in oil palm.

Keywords: abiotic stress; AP2, ERF; ethylene-responsive element; gene expression; yeast one-hybrid assay
Subjects: gene expression; transcription factor; fruit ripening; ethylene; abscisic acid; yeast one-hybrid assay; green fluorescent protein; ethylene-responsive element; dehydration-responsive element; methyl jasmonate; salicylic acid; salinity; water stress; temperature - low; oil palm; onion

Received: June 8, 2012; Accepted: January 16, 2013; Published: December 1, 2013  Show citation

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Omidvar, V., Abdullah, S.N.A., Ebrahimi, M., Ho, C.L., & Mahmood, M. (2013). Gene expression of the oil palm transcription factor EgAP2-1 during fruit ripening and in response to ethylene and ABA treatments. Biologia plantarum, 57(4), 646-654. doi: 10.1007/s10535-013-0355-8
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