biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 57:711-717, 2013 | DOI: 10.1007/s10535-013-0326-0

Ferulic acid pretreatment enhances dehydration-stress tolerance of cucumber seedlings

D. -M. Li1, Y. -X. Nie1, J. Zhang1, J. -S. Yin1, Q. Li1, X. -J. Wang1, J. -G. Bai1,*
1 State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, P.R. China

To examine whether ferulic acid (FA) could protect plants from dehydration stress and to investigate a mechanism for the protection, cucumber seedlings were pretreated with 0.5 mM FA for 2 d and then were exposed to dehydration induced by 10 % polyethylene glycol 6000. After pretreatment with FA, the activities of antioxidant enzymes (catalase, superoxide dismutase, and quaiacol peroxidase) in leaves were higher than under dehydration treatment alone which was in accordance with the increased transcript levels of respective genes. Moreover, the combination of FA pretreatment and dehydration reduced the content of superoxide radical, hydrogen peroxide, and malondialdehyde, and increased the relative water content and content of FA, proline, and soluble sugars in comparison with dehydration alone. We propose that pretreatment with FA protects cucumbers against dehydration stress by decrease of lipid peroxidation due to activation of antioxidant enzymes and by increase of proline and soluble sugar content in leaves.

Keywords: catalase; Cucumis sativus; guaiacol peroxidase; malondialdehyde; polyethylene glycol; superoxide dismutase
Subjects: ferulic acid; water stress; stress tolerance; antioxidants; catalase; peroxidase; malondialdehyde; polyethylene glycol; superoxide dismutase; proline; sugars; hydrogen peroxide; growth; glutathione reductase; relative water content; glutathione peroxidase; cucumber

Received: September 6, 2012; Accepted: January 25, 2013; Published: December 1, 2013  Show citation

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Li, D.-M., Nie, Y.-X., Zhang, J., Yin, J.-S., Li, Q., Wang, X.-J., & Bai, J.-G. (2013). Ferulic acid pretreatment enhances dehydration-stress tolerance of cucumber seedlings. Biologia plantarum, 57(4), 711-717. doi: 10.1007/s10535-013-0326-0
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    References

    1. Asada, K.: Ascorbate peroxidase - a hydrogen peroxide-scavenging enzyme in plants. - Physiol. Plant. 85: 235-241, 1992. Go to original source...
    2. Barrs, H.D., Weatherley, P.E.: A re-examination of the relative turgidity technique for estimating water deficit in leaves. - Aust. J. biol. Sci. 15: 413-428, 1962. Go to original source...
    3. Bates, L.S., Waldren, R.P., Teare, I.D.: Rapid determination of free proline for water-stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    4. Ben-Hayyim, G., Faltin, Z., Gepstein, S., Camoin, L., Strosberg, A.D., Eshdat, Y.: Isolation and characterization of salt-associated protein in Citrus. - Plant Sci. 88: 129-140, 1993. Go to original source...
    5. Bernt, E., Bergmeyer, H.U.: Inorganic peroxides. - In: Bergmeyer H.U. (ed.): Methods of Enzymatic Analysis. Pp. 2246-2248. Academic Press, New York 1974. Go to original source...
    6. Bian, S., Jiang, Y.: Reactive oxygen species, antioxidant enzyme activities and gene expression patterns in leaves and roots of Kentucky bluegrass in response to drought stress and recovery. - Sci. Hort. 120: 264-270, 2009. Go to original source...
    7. Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    8. Chaparzadeh, N., D'Amico, M.L., Khavari-Nejad, R.A., Izzo, R., Navari-Izzo, F.: Antioxidative responses of Calendula officinalis under salinity conditions. - Plant Physiol. Biochem. 42: 695-701, 2004. Go to original source...
    9. Dhindsa, R.S., Plumb-Dhindsa, P., Thorpe, T.A.: Leaf senescence: correlated with increased leaves of membrane permeability and lipid peroxidation and decreased leaves of superoxide dismutase and catalase. - J. exp. Bot. 32: 93-101, 1981. Go to original source...
    10. Doulis, A.G., Debian, N., Kingston-Smith, A.H., Foyer, C.H.: Differential localization of antioxidants in maize leaves. - Plant Physiol. 114: 1031-1037, 1997. Go to original source...
    11. Elstner, E.F., Heupel, A.: Inhibition of nitrite formation from hydroxylammonium chloride: a simple assay for superoxide dismutase. - Anal. Biochem. 70: 616-620, 1976. Go to original source...
    12. Flohe, L., Gunzler, W.A.: Assays of glutathione peroxidase. - Method. Enzymol. 105: 114-120, 1984. Go to original source...
    13. Foyer, C.H., Descourvieres, P., Kunert, K.J.: Protection against oxygen radicals: an important defence mechanism studied in transgenic plants. - Plant Cell Environ. 17: 507-523, 1994. Go to original source...
    14. Foyer, C.H., Halliwell, B.: The presence of glutathione and glutathione reductase in chloroplasts: a proposed role in ascorbic acid metabolism. - Planta 133: 21-25, 1976. Go to original source...
    15. Goicoechea, N., Merino, S., Sánchez-Díaz, M.: Arbuscular mycorrhizal fungi can contribute to maintain antioxidant and carbon metabolism in nodules of Anthyllis cytisoides L. subjected to drought. - J. Plant Physiol. 162: 27-35, 2005. Go to original source...
    16. Hoque, M.A., Banu, M.N.A., Okuma, E., Amako, K., Nakamura, Y., Shimoishi, Y., Murata, Y.: Exogenous proline and glycinebetaine increase NaCl-induced ascorbate-glutathione cycle enzyme activities, and proline improves salt tolerance more than glycinebetaine in tobacco Bright Yellow-2 suspension-cultured cells. - J. Plant Physiol. 164: 1457-1468, 2007. Go to original source...
    17. Lee, D.H., Lee, C.B.: Chilling stress-induced changes of antioxidant enzymes in the leaves of cucumber: in gel enzyme activity assays. - Plant Sci. 159: 75-85, 2000. Go to original source...
    18. Li, D.M., Zhang, J., Sun, W.J., Li, Q., Dai, A.H., Bai, J.G.: 5-Aminolevulinic acid pretreatment mitigates drought stress of cucumber leaves through altering antioxidant enzyme activity. - Sci. Hort. 130: 820-828, 2011. Go to original source...
    19. Lin, S.H., Liu, Z.J., Xu, P.L., Fang, Y.Y., Bai, J.G.: Paraquat pre-treatment increases activities of antioxidant enzymes and reduces lipid peroxidation in salt-stressed cucumber leaves. - Acta Physiol. Plant. 33: 295-304, 2011. Go to original source...
    20. Liu, J.J., Lin, S.H., Xu, P.L., Wang, X.J., Bai, J.G.: Effects of exogenous silicon on the activities of antioxidant enzymes and lipid peroxidation in chilling-stressed cucumber leaves. - Agr. Sci. China 8: 1075-1086, 2009b. Go to original source...
    21. Liu, Z.J., Zhang, X.L., Bai, J.G., Suo, B.X., Xu, P.L., Wang, L.: Exogenous paraquat changes antioxidant enzyme activities and lipid peroxidation in drought-stressed cucumber leaves. - Sci. Hort. 121: 138-143, 2009a. Go to original source...
    22. Manivannan, P., Jaleel, C.A., Somasundaram, R., Panneerselvam, R.: Osmoregulation and antioxidant metabolism in drought-stressed Helianthus annuus under triadimefon drenching. - Comp. rend. Biol. 331: 418-425, 2008. Go to original source...
    23. Naeem, M.S., Rasheed, M., Liu, D., Jin, Z.L., Ming, D.F., Yoneyama, K., Takeuchi, Y., Zhou, W.J.: 5-Aminolevulinic acid ameliorates salinity-induced metabolic, water-related and biochemical changes in Brassica napus L. - Acta Physiol. Plant. 33: 517-528, 2011. Go to original source...
    24. Pereira, G.J.G., Molina, S.M.G., Lea, P.J., Azevedo, R.A.: Activity of antioxidant enzymes in response to cadmium in Crotalaria juncea. - Plant Soil 239: 123-132, 2002. Go to original source...
    25. Ramiro, D.A., Guerreiro-Filho, O., Mazzafera, P.: Phenol contents, oxidase activities, and the resistance of coffee to the leaf miner Leucoptera coffeella. - J. chem. Ecol. 32: 1977-1988, 2006.
    26. Shan, C., He, F., Xu, G., Han, R., Liang, Z.: Nitric oxide is involved in the regulation of ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. - Biol. Plant. 56: 187-191, 2012. Go to original source...
    27. Skrebsky, E.C., Tabaldi, L.A., Pereira, L.B., Rauber, R., Maldaner, J., Cargnelutti, D., Gonçalves, J.F., Castro, G.Y., Shetinger, M.R.C., Nicoloso, F.T.: Effect of cadmium on growth, micronutrient concentration, and δ-aminolevulinic acid dehydratase and acid phosphatase activities in plants of Pfaffia glomerata. - Braz. J. Plant Physiol. 20: 285-294, 2008. Go to original source...
    28. Srinivasan, M., Sudheer, A.R., Menon, V.P.: Ferulic acid: therapeutic potential through its antioxidant property. - J. Clin. Biochem. Nutr. 40: 92-100, 2007. Go to original source...
    29. Sun, W.J., Nie, Y.X., Gao, Y., Dai, A.H., Bai, J.G.: Exogenous cinnamic acid regulates antioxidant enzyme activity and reduces lipid peroxidation in drought-stressed cucumber leaves. - Acta Physiol. Plant. 34: 641-655, 2012. Go to original source...
    30. Thapa, G., Dey, M., Sahoo, L., Panda, S.K.: An insight into the drought stress induced alterations in plants. - Biol. Plant. 55: 603-613, 2011. Go to original source...
    31. Turner, J.A., Rice, E.L.: Microbial decomposition of ferulic acid in soil. - J. chem. Ecol. 1: 41-58, 1975. Go to original source...
    32. Van den Berg, L., Zeng, Y.J.: Response of South African indigenous grass species to drought stress induced by polyethylene glycol (PEG) 6000. - S. Afr. J. Bot. 72: 284-286, 2006. Go to original source...
    33. Wang, W.B., Kim, Y.H., Lee, H.S., Kim, K.Y., Deng, X.P., Kwak, S.S.: Analysis of antioxidant enzyme activity during germination of alfalfa under salt and drought stresses. - Plant Physiol. Biochem. 47: 570-577, 2009. Go to original source...
    34. Xue, T., Hartikainen, H., Piironen, V.: Antioxidative and growth-promoting effect of selenium on senescing lettuce. - Plant Soil 237: 55-61, 2001. Go to original source...
    35. Ye, S.F., Zhou, Y.H., Sun, Y., Zou, L.Y., Yu, J.Q.: Cinnamic acid causes oxidative stress in cucumber roots, and promotes incidence of Fusarium wilt. - Environ. exp. Bot. 56: 255-262, 2006. Go to original source...
    36. Yemm, E.W., Willis, A.J.: The estimation of carbohydrates in plant extracts by anthrone. - Biochem. J. 57: 508-514, 1954. Go to original source...
    37. Zhang, M., Duan, L., Tian, X., He, Z., Li, J., Wang, B., Li, Z.: Uniconazole-induced tolerance of soybean to water deficit stress in relation to changes in photosynthesis, hormones and antioxidant system. - J. Plant Physiol. 164: 709-717, 2007. Go to original source...
    38. Zhu, Z., Wei, G., Li, J., Qian, Q., Yu, J.: Silicon alleviates salt stress and increases antioxidant enzymes activity in leaves of salt-stressed cucumber (Cucumis sativus L.). - Plant Sci. 167: 527-533, 2004. Go to original source...

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