biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:341-347, 2014 | DOI: 10.1007/s10535-014-0392-y

Applications of ascorbic acid or proline increase resistance to salt stress in barley seedlings

R. A. Agami1,*
1 Agricultural Botany Department, Faculty of Agriculture, Fayoum University, Fayoum, Egypt

The present study was carried out to examine the effects of seed soaking in 1 mM ascorbic acid (AA) or 1 mM proline on the growth, content of photosynthetic pigments and proline, relative water content, electrolyte leakage, antioxidant enzymes and leaf anatomy of Hordeum vulgare L. Giza 124 seedlings grown in greenhouse under 100 or 200 mM NaCl. The plants exposed to the NaCl stress exhibited a significant reduction in growth, relative water content, leaf photosynthetic pigments, soluble sugars, as well as alterations in leaf anatomy. However, the treatment with AA or proline ameliorated the stress generated by NaCl and improved the above mentioned parameters. NaCl increased electrolyte leakage, proline content, and activities of antioxidant enzymes (SOD, CAT, and POX). The antioxidant enzymes and leaf anatomy exhibited considerable changes in response to AA or proline application in the absence or presence of NaCl.

Keywords: carotenoids; catalase; chlorophyll; electrolyte leakege; Hordeum vulgare; leaf anatomy; NaCl; peroxidase; relative water content; superoxide dismutase
Subjects: ascorbic acid; proline; salinity; carotenoids; chlorophyll; catalase; electrolyte leakage; peroxidase; relative water content; superoxide dismutase; saccharides; leaf anatomy; barley

Received: August 1, 2013; Revised: October 10, 2013; Accepted: October 15, 2013; Published: June 1, 2014  Show citation

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Agami, R.A. (2014). Applications of ascorbic acid or proline increase resistance to salt stress in barley seedlings. Biologia plantarum, 58(2), 341-347. doi: 10.1007/s10535-014-0392-y
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