biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:375-378, 2014 | DOI: 10.1007/s10535-014-0394-9

Chloroplastic and mitochondrial GPX genes play a critical role in rice development

G. Passaia1, A. Caverzan1,2, L. S. Fonini1,2, F. E. L. Carvalho3, J. A. G. Silveira3, M. Margis-Pinheiro1,*
1 Department of Genetics, Federal University of Rio Grande do Sul, Rio Grande do Sul, Brazil
2 Biotechnology Center, Federal University of Rio Grande do Sul, Rio Grande do Sul, Brazil
3 Department of Biochemistry and Molecular Biology, Federal University of Ceará, Ceará, Brazil

Plant glutathione peroxidases (GPX) catalyze the reduction of H2O2 or organic hydroperoxides to water, mitigating the toxicity of these compounds to cells. In rice plants, the GPX gene family is composed of five members that are distributed in a range of sub-cellular compartments including cytosol, mitochondria, chloroplasts, or endoplasmic reticulum. Of these, OsGPX1 and OsGPX4 are located in mitochondria and chloroplasts, respectively. To understand the role of these GPX in rice, the effect of knockdown of OsGPX1 and OsGPX4 in rice plants was evaluated. Our data show that OsGPX4 was essential for in vitro rice regeneration because no plants were obtained from calli carrying a hairpin construct against OsGPX4. Although the knockdown of OsGPX1 did not impair plant regeneration, the plants with silenced OsGPX1 (GPX1s plants) showed reduced shoot length and a reduced number of seeds compared to the non-transformed rice plants. These results indicate that OsGPX1 and OsGPX4 are essential for redox homeostasis which leads to normal growth and development of rice.

Keywords: glutathione peroxidase; hairpin construct; in vitro regeneration; Oryza sativa; oxidative stress; seed production; transgenic plants
Subjects: mitochondrial genes; glutatione peroxidase; hairpin; in vitro culture; chloroplastic genes; transgenic plant; plant development; rice

Received: February 4, 2013; Revised: October 16, 2013; Accepted: October 18, 2013; Published: June 1, 2014  Show citation

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Passaia, G., Caverzan, A., Fonini, L.S., Carvalho, F.E.L., Silveira, J.A.G., & Margis-Pinheiro, M. (2014). Chloroplastic and mitochondrial GPX genes play a critical role in rice development. Biologia plantarum, 58(2), 375-378. doi: 10.1007/s10535-014-0394-9
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