biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:391-394, 2014 | DOI: 10.1007/s10535-014-0389-6

Antioxidative enzymes in two in vitro cultured Salicornia species in response to increasing salinity

M. Aghaleh1,*, V. Niknam2,*, H. Ebrahimzadeh1, K. Razavi3
1 Department of Biology, Faculty of Science, Razi University, Kermanshah, Iran
2 School of Biology, and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran
3 National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

The effects of salt stress on dry mass, lipid peroxidation, polyphenol and hydrogen peroxide content and activities of antioxidative enzymes were investigated in seedlings of Salicornia persica and S. europaea grown in vitro. Seeds were germinated under a broad range of NaCl concentrations (0, 100, 200, and 300 mM) on Murashige and Skoog medium for 45 d. Dry mass of both species increased at low (100 mM) salinity but decreased at higher NaCl concentrations. Malondialdehyde (MDA) content decreased at low salinity, whereas increased at 200 and 300 mM NaCl. H2O2 content in S. europaea was considerably enhanced by salinity, but it was not significantly affected in S. persica. The salt stress progressively enhanced the polyphenol content in S. persica, whereas in S. europaea, it increased with respect to the control only at higher salinities. In both species, the salinity progressively enhanced the superoxide dismutase (SOD) and peroxidase (POD) activities, whereas the CAT activity was only registered at the low salinity and the APX activity decreaseed in both species. The results indicate that S. persica exhibited a better protection mechanism against oxidative damage and it is more salt-tolerant than S. europaea.

Keywords: ascorbate peroxidase; catalase; H2O2; malondialdehyde; peroxidase; Salicornia europea; Salicornia persica; superoxide dismutase
Subjects: in vitro culture; salinity; malondialdehyde; hydrogen peroxide; polyphenols; ascorbate peroxidase; catalase; peroxidase; superoxide dismutase

Received: September 28, 2010; Revised: July 21, 2013; Accepted: July 30, 2013; Published: June 1, 2014  Show citation

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Aghaleh, M., Niknam, V., Ebrahimzadeh, H., & Razavi, K. (2014). Antioxidative enzymes in two in vitro cultured Salicornia species in response to increasing salinity. Biologia plantarum, 58(2), 391-394. doi: 10.1007/s10535-014-0389-6
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