biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 58:561-566, 2014 | DOI: 10.1007/s10535-014-0419-4

Chloroplast elongation factor BcEF-Tu responds to turnip mosaic virus infection and heat stress in non-heading Chinese cabbage

H. -T. Peng1, Y. -X. Li1, C. -W. Zhang1, Y. Li1, X. -L. Hou1,*
1 State Key Laboratory of Crop Genetics and Germplasm Enhancement/Key Laboratory of Biology and Genetic Improvement of Horticulture Crops in East China, Ministry of Agriculture/Nanjing Agricultural University, Nanjing, P.R. China

Eukaryotic elongation factor Tu has been implicated in responses to heat stress and viral infection. In this study, the turnip mosaic virus (TuMV)-response gene BcLRK01, which encodes a leucine-rich repeat receptor-like kinase, was probed using the cDNA library of TuMV-infected leaves of non-heading Chinese cabbage (Brassica campestris ssp. chinensis). The BcEF-Tu gene, which encodes chloroplast elongation factor Tu, was obtained and verified by a yeast two-hybrid system to interact with the BcLRK01 gene. TuMV infection depressed the expression of this gene, whereas a heat stress induced its expression. Overexpression of BcEF-Tu enhanced the viability of Escherichia coli transformants under the heat stress. These results demonstrate that elongation factor BcEF-Tu responded to the TuMV infection and heat stress. This is the first report on chloroplast EF-Tu in non-heading Chinese cabbage which provides a theoretical basis for the functional research of EF-Tu.

Keywords: Brassica campestris; chlorophyll fluorescence; heterologous expression; high temperature; real-time PCR; yeast two-hybrid system
Subjects: chloroplast elongation factor; temperature - high; virus infection; chlorophyll a fluorescence; yeast two-hybrid screening; gene expression; Chinese cabbage

Received: August 20, 2013; Revised: January 6, 2014; Accepted: January 29, 2014; Published: September 1, 2014  Show citation

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Peng, H.-T., Li, Y.-X., Zhang, C.-W., Li, Y., & Hou, X.-L. (2014). Chloroplast elongation factor BcEF-Tu responds to turnip mosaic virus infection and heat stress in non-heading Chinese cabbage. Biologia plantarum, 58(3), 561-566. doi: 10.1007/s10535-014-0419-4
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    References

    1. Bhadula, S.K., Elthon, T.E., Habben, J.E., Helentjaris, T.G., Jiao, S., Ristic, Z.: Heat-stress induced synthesis of chloroplast protein synthesis elongation factor (EF-Tu) in a heat-tolerant maize line. - Planta 212: 359-366, 2001. Go to original source...
    2. Blackwell, J.L., Brinton, M.A.: Translation elongation factor-1α interacts with the 3'stem-loop region of West Nile virus genomic RNA. - J. Virol. 71: 6433-6444, 1997. Go to original source...
    3. Blumenthal, T., Carmichael, G.G.: RNA replication: function and structure of Qbeta-replicase. - Annu. Rev. Biochem. 48: 525-548, 1979. Go to original source...
    4. Das, T., Mathur, M., Gupta, A.K., Janssen, G.M., Banerjee, A.K.: RNA polymerase of vesicular stomatitis virus specifically associates with translation elongation factor-1 αβγ for its activity. - Proc. nat. Acad. Sci. USA 95: 1449-1454, 1998. Go to original source...
    5. De Nova-Ocampo, M., Villegas-Sepúlveda, N., Del Angel, R.M.: Translation elongation factor-1α, La, and PTB interact with the 3' untranslated region of dengue 4 virus RNA. - Virology 295: 337-347, 2002. Go to original source...
    6. Li, W., Hu, X., Liu, J., Jiang, G., Li, O., Xing, D.: Chromosome doubling can increase heat tolerance in Lonicera japonica as indicated by chlorophyll fluorescence imaging. - Biol. Plant. 55: 279-284, 2011. Go to original source...
    7. Livak, K. J., Schmittgen, T. D.: Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method. - Methods 25: 402-408, 2001. Go to original source...
    8. Momcilovic, I., Ristic, Z.: Expression of chloroplast protein synthesis elongation factor, EF-Tu, in two lines of maize with contrasting tolerance to heat stress during early stages of plant development. - J. Plant Physiol. 164: 90-99, 2007. Go to original source...
    9. Moriarty, T., West, R., Small, G., Rao, D., Ristic, Z.: Heterologous expression of maize chloroplast protein synthesis elongation factor (EF-Tu) enhances Escherichia coli viability under heat stress. - Plant Sci. 163: 1075-1082, 2002. Go to original source...
    10. Peng, H., Li, Y., Li, Y., Hou, X.: [Response of gene BcLRK01 to TuMV infection and stress in non-heading Chinese cabbage.] - Acta bot. boreal.-occident. sin. 32: 670-675, 2012b. [In Chinese]
    11. Peng, H., Wang, L., Li, Y., Li, Y., Guan, W., Yang, Y., Xu, X., Hou, X.: Differential gene expression in incompatible interaction between turnip mosaic virus and non-heading Chinese cabbage. - Eur. J. Plant Pathol. 132: 393-406, 2012a. Go to original source...
    12. Ristic, Z., Williams, G., Yang, G., Martin, B., Fullerton, S.: Dehydration, damage to cellular membranes, and heatshock proteins in maize hybrids from different climates. - J. Plant Physiol. 149: 424-432, 1996. Go to original source...
    13. Ristic, Z., Yang, G., Martin, B., Fullerton, S.: Evidence of association between specific heat-shock protein(s) and the drought and heat tolerance phenotype in maize. - J. Plant Physiol. 153: 497-505, 1998. Go to original source...
    14. Thivierge, K., Cotton, S., Dufresne, P. J., Mathieu, I., Beauchemin, C., Ide, C., Fortin, M.G., Laliberté, J.-F.: Eukaryotic elongation factor 1A interacts with Turnip mosaic virus RNA-dependent RNA polymerase and VPg-Pro in virus-induced vesicles. - Virology 377: 216-225, 2008. Go to original source...
    15. Torii, K. U.: Leucine-rich repeat receptor kinases in plants: structure, function, and signal transduction pathways. - Int. Rev. Cytol. 234: 1-46, 2004. Go to original source...
    16. Wei, T., Huang, T.S., McNeil, J., Laliberte, J.F., Hong, J., Nelson, R.S., Wang, A.: Sequential recruitment of the endoplasmic reticulum and chloroplasts for plant potyvirus replication. - J. Virol. 84: 799-809, 2010. Go to original source...
    17. Yamaji, Y., Kobayashi, T., Hamada, K., Sakurai, K., Yoshii, A., Suzuki, M., Namba, S., Hibi, T.: In vivo interaction between Tobacco mosaic virus RNA-dependent RNA polymerase and host translation elongation factor 1A. - Virology 347: 100-108, 2006. Go to original source...
    18. Zeenko, V. V., Ryabova, L. A., Spirin, A. S., Rothnie, H. M., Hess, D., Browning, K. S., Hohn, T.: Eukaryotic elongation factor 1A interacts with the upstream pseudoknot domain in the 3' untranslated region of tobacco mosaic virus RNA. - J. Virol. 76: 5678-5691, 2002. Go to original source...

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